Linked Life Data

1340465

Source:http://linkedlifedata.com/resource/pubmed/id/1340465

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12B
pubmed:dateCreated
1993-12-1
pubmed:databankReference
pubmed:abstractText
The ets-related protein GABP alpha interacts with the four ankyrin-type (ANK) repeats of GABP beta to form a high-affinity DNA-binding complex that recognizes a site important for herpes simplex virus type I immediate early gene activation. To investigate the selectivity and specificity of the GABP complex, we have isolated two new ETS family members, termed ER81 and ER71. ER81 and GABP alpha were present in most tissues of adult mice, whereas ER71 was restricted to testis. We have compared the DNA-binding specificities of these proteins by binding site selection. GABP alpha, ER71, and ER81 recognized the common pentanucleotide DNA sequence 5'-CGGAA/T-3'. Although subtle differences were observed for nucleotide preferences flanking this pentanucleotide core, the overall similarity of the selected sequences was most striking. Given the observation that GABP alpha interaction with GABP beta requires its intact ETS domain, we further compared the ability of GABP beta to interact with other ETS proteins. GABP beta did not augment the DNA-binding activity of the highly similar ETS domains of ER81, ER71, or Ets-1. Moreover, probing of total tissue extracts with radiolabeled GABP beta demonstrated its exceedingly stringent specificity for GABP alpha. Given that the DNA-binding specificities of these ETS proteins are similar and that the protein-protein interactions between GABP beta and GABP alpha are highly specific, we conclude that the protein interactions determine the target site selection by GABP alpha.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:geneSymbol
ER71, ER81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2502-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1340465-Amino Acid Sequence, pubmed-meshheading:1340465-Animals, pubmed-meshheading:1340465-Base Sequence, pubmed-meshheading:1340465-Binding Sites, pubmed-meshheading:1340465-Blotting, Northern, pubmed-meshheading:1340465-DNA, pubmed-meshheading:1340465-DNA-Binding Proteins, pubmed-meshheading:1340465-GA-Binding Protein Transcription Factor, pubmed-meshheading:1340465-Mice, pubmed-meshheading:1340465-Molecular Sequence Data, pubmed-meshheading:1340465-Multigene Family, pubmed-meshheading:1340465-Organ Specificity, pubmed-meshheading:1340465-Protein Binding, pubmed-meshheading:1340465-Proto-Oncogene Protein c-ets-1, pubmed-meshheading:1340465-Proto-Oncogene Proteins, pubmed-meshheading:1340465-Proto-Oncogene Proteins c-ets, pubmed-meshheading:1340465-Sequence Homology, Amino Acid, pubmed-meshheading:1340465-Transcription Factors
pubmed:year
1992
pubmed:articleTitle
Specificities of protein-protein and protein-DNA interaction of GABP alpha and two newly defined ets-related proteins.
pubmed:affiliation
Howard Hughes Research Laboratories, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.