Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-12-20
pubmed:abstractText
With the aid of the indirect hemagglutination (IHA) test and IgG ELISA the antibody profile against E. histolytica in León, Nicaragua was investigated in 562 sera from individuals belonging to various age groups. The highest reactivity was invariably recorded in the age group 6-15 years where 48% were seropositive. Several sera reactive by either one of IHA and IgG ELISA were negative by the other test. The main reason for this seems to be reactivity in different Ig classes. Treatment with 2-mercaptoethanol reduced the titre level in 63 of the 66 sera tested. Immunofluorescence using an anti-IgM conjugate showed that 26 of 43 sera contained specific IgM-antibodies, indicating that also unspecific reactions are involved in the IHA test. A comparison was made between class-specific reactivity in three population groups: healthy residents, healthy cyst carriers and patients with recent or acute liver abscess. No significant difference in the prevalence of reactions above the diagnostic significance level was recorded between cyst carriers and healthy residents. However, among the cyst carriers 33% had IgA and/or IgM antibodies but no demonstrable specific IgG. Most patients with recent and all with acute liver abscess reacted significantly above the diagnostic limit in all three tests.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0188-4409
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1340309-Adolescent, pubmed-meshheading:1340309-Adult, pubmed-meshheading:1340309-Aged, pubmed-meshheading:1340309-Animals, pubmed-meshheading:1340309-Antibodies, Protozoan, pubmed-meshheading:1340309-Antigens, Protozoan, pubmed-meshheading:1340309-Child, pubmed-meshheading:1340309-Child, Preschool, pubmed-meshheading:1340309-Dysentery, Amebic, pubmed-meshheading:1340309-Entamoeba histolytica, pubmed-meshheading:1340309-Entamoebiasis, pubmed-meshheading:1340309-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:1340309-Female, pubmed-meshheading:1340309-Hemagglutination Tests, pubmed-meshheading:1340309-Humans, pubmed-meshheading:1340309-Immunoglobulin G, pubmed-meshheading:1340309-Immunoglobulin M, pubmed-meshheading:1340309-Infant, pubmed-meshheading:1340309-Liver Abscess, Amebic, pubmed-meshheading:1340309-Male, pubmed-meshheading:1340309-Middle Aged, pubmed-meshheading:1340309-Nicaragua, pubmed-meshheading:1340309-Prevalence
pubmed:year
1992
pubmed:articleTitle
Amebiasis in Nicaragua: class specific serum antibody responses.
pubmed:affiliation
University Hospital Oscar Danilo Rosales, León, Nicaragua.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't