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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1993-5-28
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pubmed:abstractText |
To investigate the relationship between Interleukin 6(IL 6) and glomerular mesangial cells (MC), the MC of BALB/c mouse were cultured and verified subsequently by anti-keratin and antidesmin antibodies. The cultured MC were divided into two parts those with Mac-1 marker and those without by flow cytometry. The IL 6 activity of cultured supernatants of MC was measured using the IL 6-dependent cell line subclone (MH 60). The results show that the mouse MC can produce IL6 and the level of IL 6 produced by MC with Mac-1 marker was higher than that without Mac-1. The RNA from the mouse glomerular mesangial cells was hybridized in dot blots with a-32pdCTP labeled IL6 cDNA probe, the dot blot autoradiograph showed the expression of IL6 mRNA gene in mouse mesangial cells.
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pubmed:language |
chi
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0376-2491
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
664-6, 702
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1338703-Animals,
pubmed-meshheading:1338703-Cells, Cultured,
pubmed-meshheading:1338703-DNA Probes,
pubmed-meshheading:1338703-Flow Cytometry,
pubmed-meshheading:1338703-Gene Expression,
pubmed-meshheading:1338703-Glomerular Mesangium,
pubmed-meshheading:1338703-Interleukin-6,
pubmed-meshheading:1338703-Mice,
pubmed-meshheading:1338703-Mice, Inbred BALB C,
pubmed-meshheading:1338703-RNA, Messenger
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pubmed:year |
1992
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pubmed:articleTitle |
[Interleukin 6 production and interleukin 6 mRNA gene expression by mouse glomerular mesangial cells].
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pubmed:affiliation |
Department of Nephrology, PLA General Hospital, Beijing.
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pubmed:publicationType |
Journal Article,
English Abstract
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