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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1993-2-26
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pubmed:abstractText |
Absorption changes following photolysis of bovine rhodopsin in mildly sonicated membrane suspensions are monitored at 25 degrees C. Difference spectra collected at 17 times between 1 microsecond and 75 ms following excitation are analyzed globally using singular value decomposition and non-linear least-squares fitting techniques. The results are not consistent with the simple scheme: Lumirhodopsin-->Metarhodopsin I<-->Metarhodopsin II, but indicate that an intermediate with a deprotonated Schiff's base is formed nearly simultaneously with metarhodopsin I upon the decay of Lumirhodopsin.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Protons,
http://linkedlifedata.com/resource/pubmed/chemical/Retinaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodopsin,
http://linkedlifedata.com/resource/pubmed/chemical/Schiff Bases,
http://linkedlifedata.com/resource/pubmed/chemical/metarhodopsins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0031-8655
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1135-44
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading | |
pubmed:year |
1992
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pubmed:articleTitle |
Photolysis of rhodopsin results in deprotonation of its retinal Schiff's base prior to formation of metarhodopsin II.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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