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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1993-2-26
|
| pubmed:abstractText |
Examination of the regulation of prodynorphin (pro-DYN) promoter activity is limited by the absence of a good cell model. The discovery of pro-DYN mRNA and derived peptides in the reproductive tract led us to examine the cellular localization and hormonal regulation of ovarian pro-DYN expression and to evaluate normal granulosa cells as a model for studying pro-DYN gene regulation. Ovarian pro-DYN mRNA levels were significantly elevated in PMSG-primed immature rats 12-24 h after receiving an ovulatory dose of hCG. Levels peaked 2 days after hCG, remained elevated throughout the ensuing pseudopregnancy, and rose again at the end of pseudopregnancy. In situ hybridization localized pro-DYN expression predominantly to granulosa and luteal cells. Transfection of primary cultures of granulosa cells revealed that the activity of the rat pro-DYN promoter [-1858 to 133 base pairs (bp)] was increased 18- to 19-fold by hCG and human FSH treatments and 7-fold by cAMP analog treatment. Deletion analysis identified a 358 bp fragment as the primary hormone-responsive sequence (-1858 to -1500 bp; containing three potential cAMP-responsive elements); its deletion resulted in severely reduced FSH responsiveness, and its ligation to hormone-unresponsive basal promoter sequences completely restored FSH responsiveness. This is an unusually distal position for cAMP-responsive elements compared to other cAMP-regulated genes. These data demonstrate specific expression of pro-DYN in granulosa and luteal cells, which is under sensitive gonadotropin regulation, and identify a distal hormone-responsive sequence within the promoter.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chorionic Gonadotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalins,
http://linkedlifedata.com/resource/pubmed/chemical/Follicle Stimulating Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropins, Equine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/preproenkephalin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0888-8809
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2244-56
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1337148-Animals,
pubmed-meshheading:1337148-Base Sequence,
pubmed-meshheading:1337148-Cells, Cultured,
pubmed-meshheading:1337148-Chorionic Gonadotropin,
pubmed-meshheading:1337148-Cyclic AMP,
pubmed-meshheading:1337148-DNA,
pubmed-meshheading:1337148-Enkephalins,
pubmed-meshheading:1337148-Female,
pubmed-meshheading:1337148-Follicle Stimulating Hormone,
pubmed-meshheading:1337148-Gene Expression Regulation,
pubmed-meshheading:1337148-Gonadotropins, Equine,
pubmed-meshheading:1337148-Granulosa Cells,
pubmed-meshheading:1337148-In Situ Hybridization,
pubmed-meshheading:1337148-Molecular Sequence Data,
pubmed-meshheading:1337148-Ovary,
pubmed-meshheading:1337148-Ovulation Induction,
pubmed-meshheading:1337148-Promoter Regions, Genetic,
pubmed-meshheading:1337148-Protein Precursors,
pubmed-meshheading:1337148-Rats,
pubmed-meshheading:1337148-Rats, Sprague-Dawley,
pubmed-meshheading:1337148-Recombinant Fusion Proteins,
pubmed-meshheading:1337148-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1337148-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1337148-Transfection
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Regulation of prodynorphin gene expression in the ovary: distal DNA regulatory elements confer gonadotropin regulation of promoter activity.
|
| pubmed:affiliation |
Division of Reproductive Biology, Oregon Regional Primate Research Center, Beaverton 97006.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|