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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6 Pt 1
|
| pubmed:dateCreated |
1993-2-3
|
| pubmed:databankReference | |
| pubmed:abstractText |
Surfactant protein C (SP-C), a hydrophobic protein of pulmonary surfactant is essential for surfactant function. Toward elucidating molecular mechanisms that mediate regulation of SP-C gene expression in rabbit lung, we isolated and characterized cDNAs encoding rabbit SP-C and studied the regulation of SP-C gene expression during fetal lung development and by adenosine 3',5'-cyclic monophosphate (cAMP) and dexamethasone in fetal lung tissues in vitro. We found that rabbit SP-C is highly homologous to SP-C of other species and is encoded by two mRNAs that differ by an insertion of 31 nucleotides in the 3' untranslated regions. SP-C mRNAs were classified into two types based on the nucleotide sequence; type I represents RNA without the 31 nucleotide insert and comprises approximately 80-90% of total SP-C mRNA content, whereas type II represents RNA containing the insert and comprises approximately 10-20% of total SP-C mRNA content. SP-C mRNAs were induced in a coordinate manner during fetal lung development and by cAMP and dexamethasone in fetal lung tissues in vitro. Southern hybridization analysis of genomic DNA suggested that SP-C mRNAs are encoded by a single gene. Polymerase [corrected] chain reaction-amplification of genomic DNA with oligonucleotide primers flanking the insertional sequence and sequence analysis of amplified DNA showed that SP-C mRNAs are produced by alternative use of 3' splice sites of intron 5 of SP-C gene.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
263
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
L634-44
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1335697-Amino Acid Sequence,
pubmed-meshheading:1335697-Animals,
pubmed-meshheading:1335697-Base Sequence,
pubmed-meshheading:1335697-Cloning, Molecular,
pubmed-meshheading:1335697-Cyclic AMP,
pubmed-meshheading:1335697-DNA,
pubmed-meshheading:1335697-Embryonic and Fetal Development,
pubmed-meshheading:1335697-Gene Expression Regulation,
pubmed-meshheading:1335697-Glucocorticoids,
pubmed-meshheading:1335697-Humans,
pubmed-meshheading:1335697-Lung,
pubmed-meshheading:1335697-Molecular Sequence Data,
pubmed-meshheading:1335697-Nucleic Acid Hybridization,
pubmed-meshheading:1335697-Oligonucleotide Probes,
pubmed-meshheading:1335697-Polymerase Chain Reaction,
pubmed-meshheading:1335697-Proteolipids,
pubmed-meshheading:1335697-Pulmonary Surfactants,
pubmed-meshheading:1335697-RNA, Messenger,
pubmed-meshheading:1335697-RNA Splicing,
pubmed-meshheading:1335697-Rabbits,
pubmed-meshheading:1335697-Species Specificity
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Rabbit surfactant protein C: cDNA cloning and regulation of alternatively spliced surfactant protein C mRNAs.
|
| pubmed:affiliation |
Department of Molecular Biology, University of Texas Health Science Center, Tyler 75710.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|