Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-1-15
pubmed:abstractText
Several serologic studies suggest that infection by hepatitis C virus (HCV) may be associated with the development of hepatocellular carcinoma (HCC). Therefore, we examined tumor tissue and/or the surrounding liver of 20 patients for viral sequences by the polymerase chain reaction (PCR). In 12 cases, liver and tumor tissues were separable for extraction. RNA was extracted from frozen tissues and used as a template for reverse transcription followed by double PCR with nested primers for the 5'-untranslated (NT) and nonstructural NS3 regions of HCV. In addition, the tissue extracts were tested by single PCR for X gene and S gene sequences of hepatitis B virus (HBV). NT region sequences of HCV were detected in the available tumor tissue of all anti-HCV-positive patients except for one. Negative (replicative) strands of HCV RNA were found in the same tissues as positive (genomic) strands at almost the same relative amounts, suggesting replication of HCV in the tumor tissue rather than contamination by HCV-positive blood. HBV X and S sequences were demonstrated in two tumors, but were absent from three tumors that were surrounded by liver tissues with HBV X sequences. One patient had nucleic acids of both viruses in tumor tissue. These observations suggest that in addition to HBV, HCV may play a role in the development of hepatocellular carcinoma.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1312054, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1312716, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1646962, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1648539, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1653272, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1656140, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1705704, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1758869, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1847790, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1848704, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1848874, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1848875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1848908, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1849654, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1963453, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-1967327, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2050320, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2117282, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2161463, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2167010, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2168552, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2169456, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2169469, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2170265, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2170712, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2170839, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2173727, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2297196, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2509915, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2572739, http://linkedlifedata.com/resource/pubmed/commentcorrection/1334635-2572740
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1271-7
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Detection of replicative hepatitis C virus sequences in hepatocellular carcinoma.
pubmed:affiliation
Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, Louisiana 70112-2699.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't