Linked Life Data

1333924

Source:http://linkedlifedata.com/resource/pubmed/id/1333924

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-1-8
pubmed:abstractText
Peripheral blood B cells from patients with systemic autoimmune disease and healthy volunteers were immortalized using EBV and the frequencies of B cell precursors that produced immunoglobulin class-specific antibodies against anti-nRNP, a specific marker for mixed connective tissue disease, were assessed using limiting dilution analysis. The frequencies of EBV-induced B cell precursors that produced IgG anti-nRNP were correlated closely with the serum titres of the corresponding autoantibodies, which indicates that B cell precursors that produced potentially pathogenic autoantibodies could be immortalized from the peripheral blood of the patients by EBV. In contrast, the frequency of EBV-induced B cell precursors that produced IgM anti-nRNP in patients with systemic autoimmune disease was comparable to that in healthy volunteers and greater than those that produced IgG and IgA anti-nRNP. Moreover, many of the clones that produced IgM antibodies against nRNP reacted with other autoantigens, such as double-stranded DNA, single-stranded DNA and rabbit IgG. These polyreactive IgM antibodies are believed to belong to the 'natural antibodies', to be coded by the germline immunoglobulin V genes, and to react with evolutionarily conserved structural cellular components, including nRNP. Our finding that nRNP is one of the target antigens for this polyreactive autoantibody may lead to the elucidation of the origin of the pathogenic IgG and IgA anti-nRNP antibodies found in sera from patients with systemic autoimmune diseases.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-1544228, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-1663752, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-1690096, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-1700467, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-2154032, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-2469441, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-2805410, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-2848890, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-2974766, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-3402531, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-3488746, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-4621694, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-6171283, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-6194895, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-6294212, http://linkedlifedata.com/resource/pubmed/commentcorrection/1333924-6335422
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0009-9104
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
415-21
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
The B cell repertoire in patients with systemic autoimmune diseases: analysis of Epstein-Barr virus (EBV)-inducible circulating precursors that produce autoantibodies against nuclear ribonucleoprotein (nRNP).
pubmed:affiliation
Division of Immunology, National Medical Centre, Tokyo, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't