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pubmed:abstractText |
Some of the properties of a high affinity Ins(1,3,4,5)P4 3-phosphatase (Km approximately 400 nM) from the soluble fraction of pig brain are presented. Several inositol polyphosphates reduced the activity of the Ins-(1,3,4,5)P4 3-phosphatase. The most effective inhibitors were Ins(1,3,4, 5,6)P5 and InsP6 with Ki-values of about 60 nM and 3 nM, respectively. We could show that at least InsP6 is a likely substrate of the Ins(1,3,4,5)P4 3-phosphatase, which degraded InsP6 with a very low reaction velocity. This 3-phosphatase may be important for the metabolism of higher phos-phorylated inositol polyphosphates.
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