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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
1992-12-30
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pubmed:abstractText |
Transcription of interleukin-6 (IL-6) gene in human HepG2 and HeLa cells was induced by treatment with interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), phorbol 12-myristate 13-acetate, or dibutyryl cyclic AMP. These agents enhanced the expression of chloramphenicol acetyltransferase (CAT) activity in cells transfected with chimeric CAT genes driven by the transcriptional regulatory regions of human IL-6 gene. Both induced and basal levels of CAT expression were severely repressed upon co-transfection of expression vectors encoding the adenoviral E1A289R or E1A243R protein. The conserved region 1 of E1A proteins was required for this activity. IL-6-CAT expression could also be induced by co-transfecting expression vectors containing cDNAs of the catalytic subunit of protein kinase A or c-jun. E1A repressed transcriptional induction by these agents as well. Similar inhibition was observed when a CAT gene driven by the NF kappa B element of the IL-6 gene was used as a reporter plasmid. In a cell line stably transfected with the E1A gene, IL-1 or TNF-alpha failed to induce IL-6 mRNA. Electrophoretic mobility shift assays were carried out with nuclear extracts of these cells using, as probes, the NF kappa B element or the multiple regulatory element of the IL-6 gene. With either probe, additional faster migrating DNA-protein complexes were formed in the extracts of E1A-expressing cells as compared with the extracts of the corresponding control cells. Experiments with NF kappa B antibody revealed differences between the different DNA-protein complexes formed in the extract of E1A-expressing cells. These observations suggest that E1A represses IL-6 gene transcription by interfering with the formation of appropriate DNA-protein complexes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus E1A Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
24886-91
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1332971-Adenovirus E1A Proteins,
pubmed-meshheading:1332971-Binding Sites,
pubmed-meshheading:1332971-Bucladesine,
pubmed-meshheading:1332971-Carcinoma, Hepatocellular,
pubmed-meshheading:1332971-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1332971-Cyclic AMP,
pubmed-meshheading:1332971-DNA,
pubmed-meshheading:1332971-Gene Expression,
pubmed-meshheading:1332971-HeLa Cells,
pubmed-meshheading:1332971-Humans,
pubmed-meshheading:1332971-Interleukin-1,
pubmed-meshheading:1332971-Interleukin-6,
pubmed-meshheading:1332971-Liver Neoplasms,
pubmed-meshheading:1332971-NF-kappa B,
pubmed-meshheading:1332971-Oligodeoxyribonucleotides,
pubmed-meshheading:1332971-Promoter Regions, Genetic,
pubmed-meshheading:1332971-Recombinant Fusion Proteins,
pubmed-meshheading:1332971-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1332971-Thymidine Kinase,
pubmed-meshheading:1332971-Transcription, Genetic,
pubmed-meshheading:1332971-Transfection,
pubmed-meshheading:1332971-Tumor Cells, Cultured,
pubmed-meshheading:1332971-Tumor Necrosis Factor-alpha
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pubmed:year |
1992
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pubmed:articleTitle |
Transcriptional repression of interleukin-6 gene by adenoviral E1A proteins.
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pubmed:affiliation |
Department of Molecular Biology, Cleveland Clinic Foundation, Ohio 44195-5285.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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