Linked Life Data

1332055

Source:http://linkedlifedata.com/resource/pubmed/id/1332055

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1992-12-23
pubmed:abstractText
We have previously reported the analysis of DdPK3, a developmentally regulated putative serine/threonine kinase that shares approximately 50% amino acid sequence identity with metazoan cAMP-dependent protein kinase A (PKA) and protein kinase C, within their catalytic domains. Cells in which the DdPK3 gene has been disrupted do not aggregate but they are able to induce aggregation-stage genes in response to cAMP pulses and the prestalk-specific ras gene DdrasD in response to high continuous levels of cAMP but will not induce prespore gene expression. In this report, we present conclusive evidence that DdPK3 encodes the catalytic subunit of the Dictyostelium PKA. DdPK3 null cells lack kinase activity that phosphorylates a PKA-specific substrate and is specifically inhibitable by recombinant cAMP-dependent protein kinase inhibitor. DdPK3 expressed in Escherichia coli has PKA activity that is inhibitable by protein kinase inhibitor. When Ddpk3 null cells are complemented with DdPK3 expressed from an actin promoter on an extrachromosomal vector (low copy number), PKA activity is restored and the cells proceed to the slug stage but will not culminate, suggesting that properly regulated PKA activity is essential for culmination. Moreover, overexpressing DdPK3 in wild-type cells on integrating vectors (high copy number) from either an actin or prespore-specific promoter results in accelerated development and the ability to form mature spores in monolayer culture in the presence of high cAMP, a developmental potential lacking in wild-type cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1310617, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1312226, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1537860, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1586944, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-16453431, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1668191, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1668648, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1670774, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1728597, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1836954, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1840893, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1852137, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1862342, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1862343, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1864510, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1917932, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1956337, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-1968413, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2010032, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2163344, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2546676, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2551673, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2656713, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2672337, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2813371, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2820963, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2846388, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2893972, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-2985966, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3010312, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3025622, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3036373, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3047011, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3076077, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3405751, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-3467359, http://linkedlifedata.com/resource/pubmed/commentcorrection/1332055-6706957
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
89
pubmed:geneSymbol
DdrasD, ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10701-5
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase.
pubmed:affiliation
Department of Biology, University of California, San Diego, La Jolla 92093-0634.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.