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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1977-3-21
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pubmed:abstractText |
Stereospecific binding sites for (-) [3H]-alprenolol, a beta-adrenergic antagonist, have been identified in guinea-pig myocardial broken cell preparations. The concentration of the sites was 0.3 pmoles per mg of protein and the dissociation constant (at 37 degrees C) 10(-8) M. A close correlation between the ability of various beta-adrenergic antagonists to compete with tracer alprenolol binding and to block the response of isoprenaline-stimulated myocardial adenylate cyclase has been found. Low affinity sites for the labelled beta-adrenergic antagonist in contrast to stereospecific sites are heat stable and do not discriminate between the (-) and the (+) forms of the beta-adrenergic antagonists. Adenylate cyclase in guinea-pig myocardial tissue is poorly stimulated by isoprenaline or 5'-guanylylimidodiphosphate. This is attributed to a high basal activity which could be lowered by a preincubation at 37 degrees C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0028-1298
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
295
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-24
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pubmed:dateRevised |
2010-3-12
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pubmed:meshHeading |
pubmed-meshheading:13314-Adenylate Cyclase,
pubmed-meshheading:13314-Adrenergic beta-Agonists,
pubmed-meshheading:13314-Adrenergic beta-Antagonists,
pubmed-meshheading:13314-Alprenolol,
pubmed-meshheading:13314-Animals,
pubmed-meshheading:13314-Binding, Competitive,
pubmed-meshheading:13314-Guinea Pigs,
pubmed-meshheading:13314-Male,
pubmed-meshheading:13314-Myocardium,
pubmed-meshheading:13314-Receptors, Adrenergic,
pubmed-meshheading:13314-Receptors, Adrenergic, beta,
pubmed-meshheading:13314-Stereoisomerism
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pubmed:year |
1976
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pubmed:articleTitle |
Beta-adrenergic receptors in guinea-pig myocardial tissue.
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pubmed:publicationType |
Journal Article,
In Vitro
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