Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1992-12-15
pubmed:abstractText
Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are over 98% similar in DNA sequence but have specific host range, antigenic, and hemagglutination (HA) properties which were located within the capsid protein gene. In vitro mutagenesis and recombination were used to prepare 16 different recombinant genomic clones, and viruses derived from those clones were analyzed for their in vitro host range, antigenic, and HA properties. The region of CPV from 59 to 91 map units determined the ability to replicate in canine cells. A complex series of interactions was observed among the individual sequence differences between 59 and 73 map units. The canine host range required that VP2 amino acids (aa) 93 and 323 both be the CPV sequence, and those two CPV sequences introduced alone into FPV greatly increased viral replication in canine cells. Changing any one of aa 93, 103, or 323 of CPV to the FPV sequence either greatly decreased replication in canine cells or resulted in an inviable plasmid. The Asn-Lys difference of aa 93 alone was responsible for the CPV-specific epitope recognized by monoclonal antibodies. An FPV-specific epitope was affected by aa 323. Amino acids 323 and 375 together determined the pH dependence of HA. Amino acids involved in the various specific properties were all around the threefold spikes of the viral particle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1316457, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1323703, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1370555, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1373202, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1532105, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1616694, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1647068, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1651227, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1845895, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1845896, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1846492, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1871965, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-1942246, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2006420, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2155508, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2171302, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2174965, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2824850, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2835857, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2836994, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-2991581, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3176341, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3257270, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3296697, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3339330, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3348000, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3376550, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3392768, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3516005, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3881765, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3942033, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3980186, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-3989914, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-4059921, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-4130570, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-4291934, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-4705382, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-516347, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6180709, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6194613, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6250432, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6254399, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6264106, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6275684, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6306048, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6602221, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-6602222, http://linkedlifedata.com/resource/pubmed/commentcorrection/1331498-726266
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6858-67
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed-meshheading:1331498-Animals, pubmed-meshheading:1331498-Dogs, pubmed-meshheading:1331498-Cats, pubmed-meshheading:1331498-Models, Molecular, pubmed-meshheading:1331498-Base Sequence, pubmed-meshheading:1331498-Amino Acid Sequence, pubmed-meshheading:1331498-X-Ray Diffraction, pubmed-meshheading:1331498-Virus Replication, pubmed-meshheading:1331498-Cell Line, pubmed-meshheading:1331498-Antigens, Viral, pubmed-meshheading:1331498-Molecular Sequence Data, pubmed-meshheading:1331498-Hemagglutination, Viral, pubmed-meshheading:1331498-Protein Structure, Secondary, pubmed-meshheading:1331498-Genome, Viral, pubmed-meshheading:1331498-Cloning, Molecular, pubmed-meshheading:1331498-Capsid, pubmed-meshheading:1331498-Plasmids, pubmed-meshheading:1331498-Parvoviridae, pubmed-meshheading:1331498-Oligodeoxyribonucleotides
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