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rdf:type |
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lifeskim:mentions |
umls-concept:C0205314,
umls-concept:C0220781,
umls-concept:C0337112,
umls-concept:C0679622,
umls-concept:C0814999,
umls-concept:C1335439,
umls-concept:C1513380,
umls-concept:C1514873,
umls-concept:C1524075,
umls-concept:C1883254,
umls-concept:C2347338
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pubmed:issue |
11
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pubmed:dateCreated |
1992-12-21
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pubmed:abstractText |
Self-tolerance is mainly established through clonal deletion of autoreactive T cells during thymic differentiation. The mechanisms by which deletion is achieved are poorly understood. Here we use a specific polymerase chain reaction-based system to characterize DNA fragmentation and show that after in vivo treatment of neonatal mice with staphylococcus enterotoxin B, selective apoptosis of V beta 8+ thymocytes occurs. This process precedes detectable deletion of V beta 8+ cells as determined by phenotypic analysis. Moreover, in vivo administration of cycloheximide and, to a lesser extent, actinomycin D, inhibits apoptosis of staphylococcus enterotoxin B specific thymocytes. Thus, macromolecular synthesis is a requirement for negative selection.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
149
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3550-3
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1331238-Animals,
pubmed-meshheading:1331238-Antigens, Bacterial,
pubmed-meshheading:1331238-Apoptosis,
pubmed-meshheading:1331238-Base Sequence,
pubmed-meshheading:1331238-Cycloheximide,
pubmed-meshheading:1331238-DNA Damage,
pubmed-meshheading:1331238-Dactinomycin,
pubmed-meshheading:1331238-Enterotoxins,
pubmed-meshheading:1331238-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1331238-Immune Tolerance,
pubmed-meshheading:1331238-Mice,
pubmed-meshheading:1331238-Mice, Inbred Strains,
pubmed-meshheading:1331238-Molecular Sequence Data,
pubmed-meshheading:1331238-Oligodeoxyribonucleotides,
pubmed-meshheading:1331238-Polymerase Chain Reaction,
pubmed-meshheading:1331238-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:1331238-Staphylococcus aureus
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pubmed:year |
1992
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pubmed:articleTitle |
Negative selection of thymocytes. A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis.
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pubmed:affiliation |
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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