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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1992-12-21
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pubmed:abstractText |
Continuous cell lines were assessed for use for rapid human cytomegalovirus (HCMV) detection procedures combining tissue culture, centrifugation, and immediate early antigen (IEA) immunostaining. Human cells (MRC-5 embryonic fibroblasts, U-373MG astrocytoma cells, differentiated teratocarcinoma (Tera-2) cells), murine cells (BALB/c-3T3 and Y-1 cells), BHK21 hamster cells, and mink lung (ML) cells were first inoculated with HCMV laboratory strain. IEA synthesizing cells were detected by immunoperoxidase assay using a monoclonal antibody. ML cells and differentiated Tera-2 cells exhibited more positive cells than MRC-5 cells. BHK21, and MRC-5 cells were equivalent in sensitivity whereas U-373MG, BALB/c-3T3, and Y-1 cells had only reduced IEA positive cells. When 63 urine specimens were inoculated onto MRC-5, ML and differentiated Tera-2 cells, 20 (31.7%) were positive in MRC-5 cells versus 18 (28.5%) in ML or Tera-2 cells. Moreover, greater numbers of infected cells were detected in MRC-5 cells than in these two cell lines. MRC-5 cells were superior for detection of HCMV in clinical samples by centrifugation cultures.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0166-0934
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
39
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
311-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1331154-Animals,
pubmed-meshheading:1331154-Antigens, Viral,
pubmed-meshheading:1331154-Cell Division,
pubmed-meshheading:1331154-Cell Line,
pubmed-meshheading:1331154-Cricetinae,
pubmed-meshheading:1331154-Cytomegalovirus,
pubmed-meshheading:1331154-Humans,
pubmed-meshheading:1331154-Immediate-Early Proteins,
pubmed-meshheading:1331154-Mice,
pubmed-meshheading:1331154-Species Specificity,
pubmed-meshheading:1331154-Tumor Cells, Cultured,
pubmed-meshheading:1331154-Urine
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pubmed:year |
1992
|
pubmed:articleTitle |
Comparison of MRC-5 and continuous cell lines for detection of cytomegalovirus in centrifugation cultures.
|
pubmed:affiliation |
Service de Bactériologie-Virologie, Hôpital Saint-Louis, Université Paris VII, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
|