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rdf:type |
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lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0010531,
umls-concept:C0034987,
umls-concept:C0056695,
umls-concept:C0127400,
umls-concept:C0242210,
umls-concept:C0441712,
umls-concept:C0851827,
umls-concept:C1701901,
umls-concept:C1707524,
umls-concept:C1948023
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pubmed:issue |
33
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pubmed:dateCreated |
1992-12-22
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pubmed:abstractText |
Catecholamines stimulate proopiomelanocortin (POMC) gene expression in corticotrope cells, but the molecular mechanisms of these effects are not known. While beta-adrenergic receptors stimulate the protein kinase A (PKA) system, the POMC promoter does not have classical cAMP-response elements (CREs). Therefore, we investigated the induction of the c-fos protooncogen, previously shown to increase POMC transcription in AtT20 cells. In this corticotrope-derived cell line, we show that activation of beta-receptors with isoprenaline (Iso) induces a transient rise in c-fos mRNA levels. Gel mobility shift assays with a labeled AP1 consensus sequence (TGACTCA) showed induction of specific binding activity after Iso treatment. Cotransfection experiments with dominant inhibitory PKA mutants and reporter genes containing c-fos promoter sequences showed that c-fos induction by Iso is entirely dependent on a functional PKA activity. Furthermore, we show that beta-receptor induction of c-fos in corticotrophs is mediated by at least two distinct cAMP-responsive sequences. cAMP regulatory element binding (CREB)-dependent induction is observed on the CRE located at -60 bp on the c-fos promoter. A region located in the vicinity of the dyad symetry element (-290) is also found to mediate tissue-specific cAMP induction. Transcriptional activation by this site, although sensitive to PKA antagonism, is not blocked by CREB mutants.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Pro-Opiomelanocortin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
267
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pubmed:geneSymbol |
c-fos
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23520-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1331087-Animals,
pubmed-meshheading:1331087-Base Sequence,
pubmed-meshheading:1331087-Cell Nucleus,
pubmed-meshheading:1331087-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1331087-Cyclic AMP,
pubmed-meshheading:1331087-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:1331087-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:1331087-Genes, fos,
pubmed-meshheading:1331087-Humans,
pubmed-meshheading:1331087-Isoproterenol,
pubmed-meshheading:1331087-Kinetics,
pubmed-meshheading:1331087-Metallothionein,
pubmed-meshheading:1331087-Mice,
pubmed-meshheading:1331087-Molecular Sequence Data,
pubmed-meshheading:1331087-Oligodeoxyribonucleotides,
pubmed-meshheading:1331087-Pituitary Neoplasms,
pubmed-meshheading:1331087-Pro-Opiomelanocortin,
pubmed-meshheading:1331087-Promoter Regions, Genetic,
pubmed-meshheading:1331087-Protein Kinases,
pubmed-meshheading:1331087-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:1331087-RNA, Messenger,
pubmed-meshheading:1331087-Receptors, Adrenergic, beta,
pubmed-meshheading:1331087-Sequence Deletion,
pubmed-meshheading:1331087-Transfection,
pubmed-meshheading:1331087-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Beta-adrenergic stimulation of cFOS via protein kinase A is mediated by cAMP regulatory element binding protein (CREB)-dependent and tissue-specific CREB-independent mechanisms in corticotrope cells.
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pubmed:affiliation |
Institut de Physiologie et de Chimie Biologique, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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