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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1992-11-17
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pubmed:abstractText |
We have examined the expression of the recombination activating gene RAG-1 by in situ hybridization to thymi from mice bearing transgenes for the T-cell receptor (TCR) alpha chain, TCR beta chain, or both TCR alpha and beta chains. RAG-1 transcription was found in the thymic cortex of transgenic mice carrying a single TCR alpha- or TCR beta-chain transgene, comparable to normal mice. However, RAG-1 transcription was strikingly reduced in the thymic cortex from transgenic mice carrying both TCR alpha- and beta-chain genes and expressing major histocompatibility complex (MHC) class I (H-2b) molecules necessary for positive selection of the transgenic TCR. In contrast, thymi of transgenic mice also carrying both TCR alpha- and beta-chain genes but expressing MHC molecules (H-2d) that did not positively select the transgenic TCR displayed high levels of RAG-1 transcription. The low thymic RAG-1 expression coincided with high transgenic TCR alpha-chain surface expression and with inhibition of endogenous TCR alpha-chain rearrangement. Our findings suggest that binding of the TCR to self MHC molecules during positive selection down-regulates RAG-1 transcription in cortical thymocytes and thereby prevents further TCR alpha-chain rearrangements.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1311260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1316241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1386545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1547487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1547488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1655004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1696684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1716213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1831564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1836010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1868548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1968840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-1975427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2138558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2156936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2160502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2188673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2318248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2360047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2521375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2524380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2562159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2573841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2598259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2668013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2673539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2970670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-2972613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-3043226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-3258191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-3260351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-3262424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-3518750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-4831907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-6206178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1329099-6254664
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RAG-1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:geneSymbol |
RAG-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9529-33
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1329099-Animals,
pubmed-meshheading:1329099-Antigens, CD4,
pubmed-meshheading:1329099-Antigens, CD8,
pubmed-meshheading:1329099-Base Sequence,
pubmed-meshheading:1329099-Cell Differentiation,
pubmed-meshheading:1329099-Cell Membrane,
pubmed-meshheading:1329099-Gene Expression,
pubmed-meshheading:1329099-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1329099-Homeodomain Proteins,
pubmed-meshheading:1329099-In Situ Hybridization,
pubmed-meshheading:1329099-Mice,
pubmed-meshheading:1329099-Mice, Transgenic,
pubmed-meshheading:1329099-Molecular Sequence Data,
pubmed-meshheading:1329099-Oligodeoxyribonucleotides,
pubmed-meshheading:1329099-Proteins,
pubmed-meshheading:1329099-RNA, Messenger,
pubmed-meshheading:1329099-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:1329099-T-Lymphocytes,
pubmed-meshheading:1329099-Thymus Gland
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pubmed:year |
1992
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pubmed:articleTitle |
Engagement of the T-cell receptor during positive selection in the thymus down-regulates RAG-1 expression.
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pubmed:affiliation |
Institute of Experimental Immunology, University of Zurich, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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