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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6394
|
| pubmed:dateCreated |
1992-11-16
|
| pubmed:abstractText |
Synapsin I is a synaptic vesicle-associated phosphoprotein that is involved in the modulation of neurotransmitter release. Ca2+/calmodulin-dependent protein kinase II, which phosphorylates two sites in the carboxy-terminal region of synapsin I, causes synapsin I to dissociate from synaptic vesicles and increases neurotransmitter release. Conversely, the dephosphorylated form of synapsin I, but not the form phosphorylated by Ca2+/calmodulin-dependent protein kinase II, inhibits neurotransmitter release. The amino-terminal region of synapsin I interacts with membrane phospholipids, whereas the C-terminal region binds to a protein component of synaptic vesicles. Here we demonstrate that the binding of the C-terminal region of synapsin I involves the regulatory domain of a synaptic vesicle-associated form of Ca2+/calmodulin-dependent protein kinase II. Our results indicate that this form of the kinase functions both as a binding protein for synapsin I, and as an enzyme that phosphorylates synapsin I and promotes its dissociation from the vesicles.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0028-0836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
359
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
417-20
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:1328883-Amino Acid Sequence,
pubmed-meshheading:1328883-Animals,
pubmed-meshheading:1328883-Brain,
pubmed-meshheading:1328883-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:1328883-Chromatography, High Pressure Liquid,
pubmed-meshheading:1328883-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:1328883-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1328883-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:1328883-Molecular Sequence Data,
pubmed-meshheading:1328883-Phosphorylation,
pubmed-meshheading:1328883-Protein Kinases,
pubmed-meshheading:1328883-Rats,
pubmed-meshheading:1328883-Receptors, Neurotransmitter,
pubmed-meshheading:1328883-Substrate Specificity,
pubmed-meshheading:1328883-Synapsins,
pubmed-meshheading:1328883-Synaptic Vesicles
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I.
|
| pubmed:affiliation |
Institute of Human Physiology, University of Modena, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|