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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1992-11-3
|
| pubmed:abstractText |
A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha 2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0910-5050
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
83
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
871-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1328123-Aged,
pubmed-meshheading:1328123-Aged, 80 and over,
pubmed-meshheading:1328123-Base Sequence,
pubmed-meshheading:1328123-Carcinoma, Hepatocellular,
pubmed-meshheading:1328123-Cell Division,
pubmed-meshheading:1328123-Chromosomes, Human, Pair 1,
pubmed-meshheading:1328123-Chromosomes, Human, Pair 17,
pubmed-meshheading:1328123-Chromosomes, Human, Pair 20,
pubmed-meshheading:1328123-Culture Techniques,
pubmed-meshheading:1328123-Gene Rearrangement,
pubmed-meshheading:1328123-Hepacivirus,
pubmed-meshheading:1328123-Humans,
pubmed-meshheading:1328123-In Situ Hybridization,
pubmed-meshheading:1328123-Karyotyping,
pubmed-meshheading:1328123-Kinetics,
pubmed-meshheading:1328123-Liver Neoplasms,
pubmed-meshheading:1328123-Male,
pubmed-meshheading:1328123-Microscopy, Electron,
pubmed-meshheading:1328123-Molecular Sequence Data,
pubmed-meshheading:1328123-Oligodeoxyribonucleotides,
pubmed-meshheading:1328123-Oligonucleotides, Antisense,
pubmed-meshheading:1328123-Polymerase Chain Reaction,
pubmed-meshheading:1328123-RNA, Viral,
pubmed-meshheading:1328123-Tumor Cells, Cultured,
pubmed-meshheading:1328123-Tumor Markers, Biological
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
New hepatocellular carcinoma cell line SUHC-1 established from a patient with hepatitis C virus RNA in serum.
|
| pubmed:affiliation |
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto.
|
| pubmed:publicationType |
Journal Article,
Case Reports,
Research Support, Non-U.S. Gov't
|