Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1992-11-2
pubmed:databankReference
pubmed:abstractText
Transcription initiation at CytR regulated promoters in Escherichia coli is controlled by a combinatorial regulatory system in which the cAMP receptor protein (CRP) functions as both an activator and a co-repressor. By combining genetic studies and footprinting analyses, we demonstrate that regulated expression of the CytR controlled cdd promoter requires three CRP-binding sites: a high affinity site (CRP-1) and two overlapping low affinity sites (CRP-2 and CRP-3) centred at positions -41, -91 and -93, respectively. In the absence of CytR, cAMP-CRP interacts at one set of sites (CRP-1 and CRP-2) and both of these binding sites are required for full promoter activation. In the presence of CytR, however, the two regulators bind cooperatively to cddP forming a nucleoprotein complex in which cAMP-CRP binds to CRP-1 and CRP-3 and CytR occupies the sequence between these sites. Thus, association of the two regulators involves a repositioning of the cAMP-CRP complex. Moreover, mutant cdd promoters in which CRP-2 and CRP-3 have been deleted are partially regulated by CytR, and cAMP-CRP and CytR still bind cooperatively to these promoters. These findings provide clues to an understanding of how cAMP-CRP and CytR interact at a structurally diverse set of promoters.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1645411, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1645842, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1647022, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1649947, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1650341, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1653449, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1655726, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1661372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1715855, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1824723, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1824874, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1913806, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-1962841, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-204861, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-212715, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2162467, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2167178, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2170326, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2173826, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2237403, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2259621, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2512122, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2575702, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2673007, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-2844254, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-3001650, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-3019678, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-3289753, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-3547140, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-6098531, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-6295879, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-6323164, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-6329724, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-6372090, http://linkedlifedata.com/resource/pubmed/commentcorrection/1327747-6455590
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3635-43
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
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