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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-10-30
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pubmed:abstractText |
A detailed comparison was made of the concentration dependence of translation inhibition by phosphorothioate and phosphodiester oligodeoxynucleotides of the same anti-beta-globin sequence in cell-free systems using beta-globin mRNA and unrelated mRNAs as controls. The results confirm that at low concentrations the phosphorothioate oligomer is more potent as an antisense compound, while at higher concentration (greater than 4 microM) it exhibits more nonspecific inhibition than the phosphodiester oligomer for RNase H-mediated translation inhibition.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1050-5261
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1327332-Base Sequence,
pubmed-meshheading:1327332-Cell-Free System,
pubmed-meshheading:1327332-Escherichia coli,
pubmed-meshheading:1327332-Globins,
pubmed-meshheading:1327332-Humans,
pubmed-meshheading:1327332-Molecular Sequence Data,
pubmed-meshheading:1327332-Oligonucleotides, Antisense,
pubmed-meshheading:1327332-Protein Biosynthesis,
pubmed-meshheading:1327332-RNA, Messenger,
pubmed-meshheading:1327332-Ribonuclease H,
pubmed-meshheading:1327332-Thionucleotides
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pubmed:year |
1992
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pubmed:articleTitle |
Translation inhibition by phosphorothioate oligodeoxynucleotides in cell-free systems.
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pubmed:affiliation |
Pharmacology Department, Georgetown University Medical Center, Washington DC.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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