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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-10-22
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pubmed:abstractText |
The expression of a regulatory subunit of calcineurin (CaN beta) during rat spermatogenesis was examined in rat testes using a monoclonal antibody Va1. Results showed that a testis-specific isoform of CaN beta was expressed only 3 weeks after birth, when meiosis begins, and increased in amount depending on the maturation of spermatogenesis. The matured sperm, which consists of only post-meiotic cells, is most likely to have only the testis-specific isoform of CaN beta. The brain type isoform of CaN beta was not detected in rat sperm. Immunoblot analysis of testes from different rodent species by a monoclonal antibody Va1 showed that all rodent species examined had their own homologues corresponding to a testis-specific isoform of CaN beta in rats, although they showed distinctively different molecular weights on SDS-PAGE compared to the testis-specific isoform in rats. Each homologue was shown to be specifically expressed in post-meiotic phase of spermatogenesis, as was seen in rats.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
828-31
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1326957-Aging,
pubmed-meshheading:1326957-Animals,
pubmed-meshheading:1326957-Antibodies, Monoclonal,
pubmed-meshheading:1326957-Brain,
pubmed-meshheading:1326957-Calcineurin,
pubmed-meshheading:1326957-Calmodulin-Binding Proteins,
pubmed-meshheading:1326957-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1326957-Guinea Pigs,
pubmed-meshheading:1326957-Immunoblotting,
pubmed-meshheading:1326957-Isoenzymes,
pubmed-meshheading:1326957-Macromolecular Substances,
pubmed-meshheading:1326957-Male,
pubmed-meshheading:1326957-Meiosis,
pubmed-meshheading:1326957-Mice,
pubmed-meshheading:1326957-Molecular Weight,
pubmed-meshheading:1326957-Phosphoprotein Phosphatases,
pubmed-meshheading:1326957-Rats,
pubmed-meshheading:1326957-Spermatogenesis,
pubmed-meshheading:1326957-Testis
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pubmed:year |
1992
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pubmed:articleTitle |
The evidence for post-meiotic expression of a testis-specific isoform of a regulatory subunit of calcineurin using a monoclonal antibody.
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pubmed:affiliation |
Department of Physiology, Kagawa Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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