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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1992-10-22
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pubmed:databankReference |
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pubmed:abstractText |
We recently reported the molecular cloning of a cytotoxic granule-associated RNA-binding protein designated TIA-1. The ability of recombinant TIA-1 to induce DNA fragmentation in permeabilized cells suggested that this protein is the granule component responsible for inducing apoptosis in cytolytic lymphocyte (CTL) targets. Here we report the characterization of a cDNA encoding a TIA-1-related protein designated TIAR. The deduced amino acid sequence of TIAR reveals it to be a 42-kDa protein possessing three RNA-binding domains and a carboxyl-terminal auxiliary domain. Although the RNA-binding domains of TIA-1 and TIAR share greater than 85% amino acid homology, their carboxyl-terminal auxiliary domains are only 51% homologous. The carboxyl terminus of TIAR contains a lysosome-targeting motif, indicating that TIAR is probably a cytotoxic granule-associated protein. Like TIA-1, purified recombinant TIAR induced DNA fragmentation in permeabilized target cells. Although immunoblotting analysis of post-nuclear supernatants revealed TIA-1 protein to be restricted to CTLs, PCR analysis revealed the expression of TIA-1 and TIAR mRNA transcripts in a wide variety of cell types. Our data suggest that the granules of CTLs contain at least two candidate nucleolysins involved in CTL killing.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-1732416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-1910674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-1934064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-1939168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2022921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2104899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2188665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2417234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2450162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2470643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2521375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2538546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2683611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2783478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2787157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2788710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-2794864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-3076768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-3284289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-3494953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-3657801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-3904772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-6245367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-6312454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-6347870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326761-6694911
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microbial Collagenase,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(A)-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TIA1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8681-5
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:1326761-Amino Acid Sequence,
pubmed-meshheading:1326761-Antibodies, Monoclonal,
pubmed-meshheading:1326761-Base Sequence,
pubmed-meshheading:1326761-Blotting, Southern,
pubmed-meshheading:1326761-Cell Nucleus,
pubmed-meshheading:1326761-Cloning, Molecular,
pubmed-meshheading:1326761-Consensus Sequence,
pubmed-meshheading:1326761-Cytotoxicity, Immunologic,
pubmed-meshheading:1326761-DNA,
pubmed-meshheading:1326761-DNA Damage,
pubmed-meshheading:1326761-Gene Expression,
pubmed-meshheading:1326761-Genes,
pubmed-meshheading:1326761-Humans,
pubmed-meshheading:1326761-Membrane Proteins,
pubmed-meshheading:1326761-Microbial Collagenase,
pubmed-meshheading:1326761-Molecular Sequence Data,
pubmed-meshheading:1326761-Poly(A)-Binding Proteins,
pubmed-meshheading:1326761-Proteins,
pubmed-meshheading:1326761-RNA, Messenger,
pubmed-meshheading:1326761-RNA-Binding Proteins,
pubmed-meshheading:1326761-Sequence Alignment
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pubmed:year |
1992
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pubmed:articleTitle |
Identification and functional characterization of a TIA-1-related nucleolysin.
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pubmed:affiliation |
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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