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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1992-10-22
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pubmed:abstractText |
In spite of the fact that a DNA helicase is clearly required for the predominantly leading-strand synthesis occurring during mammalian mtDNA replication, no such activity has heretofore been identified. We report the characterization of a mammalian mitochondrial DNA helicase isolated from bovine brain tissue. The sucrose gradient-purified mitochondria in which the activity was detected had less than 1 part in 2500 nuclear contamination according to Western blot analysis using nuclear- and mitochondrial-specific probes. Mitochondrial protein fractionation by DEAE-Sephacel chromatography yielded a DNA helicase activity dependent upon hydrolysis of ATP or dATP but not other NTPs or dNTPs. The mitochondrial helicase unwound 15- and 20-base oligonucleotides but was unable to unwind 32-base or longer oligonucleotides, and the polarity of the unwinding is 3'-to-5' with respect to the single-stranded portion of the partial duplex DNA substrate. This direction of unwinding would place the bovine mitochondrial helicase on the template strand ahead of DNA polymerase gamma during mtDNA replication, a situation analogous to that of the Rep helicase of Escherichia coli during leading-strand DNA synthesis of certain bacteriophages.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1064029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1176550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-138139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-147874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1649452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1700273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1702201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1710980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1809353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1849081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-1974896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2160269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2164680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2165383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2166690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-221901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2315045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2461720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2684415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2716784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2826443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2826446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-289452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2959842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-2995099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-3003910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-3007474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-3019672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-3038170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-324521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-3745193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-3779841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-4212385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-4473554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-4501588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-4509344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-4612520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-5289384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-537081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-604505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-604507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6122686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6209285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6224217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6288720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6310571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6311667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6315716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-6978464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1326759-7120390
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8562-6
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1326759-Adenosine Triphosphate,
pubmed-meshheading:1326759-Animals,
pubmed-meshheading:1326759-Base Sequence,
pubmed-meshheading:1326759-Brain,
pubmed-meshheading:1326759-Cattle,
pubmed-meshheading:1326759-Cell Fractionation,
pubmed-meshheading:1326759-DNA, Mitochondrial,
pubmed-meshheading:1326759-DNA Helicases,
pubmed-meshheading:1326759-DNA Replication,
pubmed-meshheading:1326759-Deoxyadenine Nucleotides,
pubmed-meshheading:1326759-Mitochondria,
pubmed-meshheading:1326759-Molecular Sequence Data,
pubmed-meshheading:1326759-Oligodeoxyribonucleotides,
pubmed-meshheading:1326759-Substrate Specificity
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pubmed:year |
1992
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pubmed:articleTitle |
DNA helicase from mammalian mitochondria.
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pubmed:affiliation |
Department of Immunology, University of Florida, Gainesville 32610.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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