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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
1992-10-20
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pubmed:abstractText |
L-Type cardiac Ca2+ channels expressed in Xenopus oocyte were studied following rat heart ribonucleic acid, messenger (mRNA) injection. We demonstrate that exogenous Ca2+ channels are sensitive to intracellular regulation by protein kinase C (PKC). This was performed by using two types of PKC activators [phorbol esters and a structural analogue of diacyl-glycerol (DAG)] and a specific peptidic inhibitor. Ca2+ channel modulation resulted in an initial increase of the inward current, without any modification of the voltage-dependent properties, and a second delayed phase, specifically observed with phorbol esters, characterized by a progressive decrease in current amplitude. Concomitantly, a reduction of membrane capacitance, reflecting a reduction of the total membrane surface area, was observed. We suggest that this phenomenon underlies the irreversible decrease of the expressed Ba2+ current via sequestration of Ca2+ channels and/or PKC. We also demonstrate that regulation of cardiac mRNA-directed Ca2+ channels by PKC activators was strictly dependent on intracellular Ca2+ concentration, and was partially additive with cyclic-adenosine-monophosphate-(cAMP) dependent regulation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol 12,13-Dibutyrate,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0031-6768
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
421
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-55
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1326746-Animals,
pubmed-meshheading:1326746-Calcium Channels,
pubmed-meshheading:1326746-Cyclic AMP,
pubmed-meshheading:1326746-Diglycerides,
pubmed-meshheading:1326746-Down-Regulation,
pubmed-meshheading:1326746-Electrophysiology,
pubmed-meshheading:1326746-Enzyme Activation,
pubmed-meshheading:1326746-Myocardium,
pubmed-meshheading:1326746-Oocytes,
pubmed-meshheading:1326746-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:1326746-Protein Kinase C,
pubmed-meshheading:1326746-RNA, Messenger,
pubmed-meshheading:1326746-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1326746-Xenopus
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pubmed:year |
1992
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pubmed:articleTitle |
Protein kinase C regulation of cardiac calcium channels expressed in Xenopus oocytes.
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pubmed:affiliation |
Centre de Recherches de Biochimie Macromoleculaire, CNRS UPR 9008, INSERM U 249, Montpellier, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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