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rdf:type |
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lifeskim:mentions |
umls-concept:C0027950,
umls-concept:C0028128,
umls-concept:C0033268,
umls-concept:C0035028,
umls-concept:C0038836,
umls-concept:C0068355,
umls-concept:C0225336,
umls-concept:C0439851,
umls-concept:C0441472,
umls-concept:C1521761,
umls-concept:C1552596,
umls-concept:C1947931
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pubmed:issue |
3
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pubmed:dateCreated |
1992-10-13
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pubmed:abstractText |
Nitric oxide provokes vasodilation and inhibits platelet aggregation. We examined the effect of nitric oxide on superoxide anion production by three sources: activated intact neutrophils, xanthine oxidase/hypoxanthine, and the NADPH oxidase. Nitric oxide significantly inhibited the generation of superoxide anion by neutrophils exposed to either FMLP (10(-7)M) or PMA (150 ng/ml) (IC50 = 30 microM). To determine whether the effect of nitric oxide on the respiratory burst was due to simple scavenging of O2+, kinetic studies that compared effects on neutrophils and the cell-free xanthine oxidase system were performed. Nitric oxide inhibited O2+ produced by xanthine oxidase only when added simultaneously with substrate, consistent with the short half-life of NO in oxygenated solution. In contrast, the addition of nitric oxide to neutrophils 20 min before FMLP resulted in the inhibition of O2+ production, which suggests formation of a stable intermediate. The effect of nitric oxide on the cell-free NADPH oxidase superoxide-generating system was also examined: The addition of NO before arachidonate activation (t = -6 min) significantly inhibited superoxide anion production. Nitric oxide did not inhibit O2+ when added at NADPH initiation (t = 0). Treatment of the membrane but not cytosolic component of the oxidase was sufficient to inhibit O2+ generation. The data suggest that nitric oxide inhibits neutrophil O2+ production via direct effects on membrane components of the NADPH oxidase. This action must occur before the assembly of the activated complex.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-13358864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1645316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1660188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1675786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1692159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1700301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1702214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1704888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1720572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1847917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1898732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-1906890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-193872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2077935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2110626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2124251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2138998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2154753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-216707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2172242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2497467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2540223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2541713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2547247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2548208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2584226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2777766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2819872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2827173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-2997778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-3131684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-3260776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-3745437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-3745439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-3918593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-4151956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-4179068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-4338124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6105889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6111365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6115052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6135148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6255012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6261830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6336905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-6871231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-7015032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-7029133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-7142287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1325992-7329277
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1116-21
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1325992-Calcium,
pubmed-meshheading:1325992-Cell Degranulation,
pubmed-meshheading:1325992-Cells, Cultured,
pubmed-meshheading:1325992-Humans,
pubmed-meshheading:1325992-NADH, NADPH Oxidoreductases,
pubmed-meshheading:1325992-NADPH Oxidase,
pubmed-meshheading:1325992-Neutrophils,
pubmed-meshheading:1325992-Nitric Oxide,
pubmed-meshheading:1325992-Superoxides,
pubmed-meshheading:1325992-Xanthine Oxidase
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pubmed:year |
1992
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pubmed:articleTitle |
Nitric oxide, an endothelial cell relaxation factor, inhibits neutrophil superoxide anion production via a direct action on the NADPH oxidase.
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