|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1992-10-13
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
CCAAT/enhancer-binding protein (C/EBP) is expressed in certain cell types including hepatocytes and adipocytes. In order to understand the mechanisms that control the expression of the mouse C/EBP gene in the liver as well as in adipocytes, we have studied both the endogenous gene and transfected C/EBP gene constructs. The initiation site of transcription was identified and a strong liver-specific DNase-I hypersensitive site located at -3 kb, which does not appear to contribute functionally to the regulation of the gene in a variety of either transiently or stably transfected cells with constructs which include sequences up to 6-kb upstream of the transcription start. C/EBP gene expression during the transition from preadipocytes to adipocytes was shown to be controlled at the level of transcription. However, adipocytes stably transfected with constructs that include -3.3 kb upstream of the C/EBP gene do not express the reporter genes in a differentiation-specific manner. We detected several DNA-binding proteins that interact with the upstream sites of the C/EBP gene. Those include two labile and two heat-stable site-specific DNA-binding proteins that are present in nuclear extracts from several tissues and cultured cell lines.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0014-2956
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
208
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
501-9
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:1325913-Adipose Tissue,
pubmed-meshheading:1325913-Animals,
pubmed-meshheading:1325913-Base Sequence,
pubmed-meshheading:1325913-Binding Sites,
pubmed-meshheading:1325913-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:1325913-Carcinoma, Hepatocellular,
pubmed-meshheading:1325913-Cell Differentiation,
pubmed-meshheading:1325913-DNA-Binding Proteins,
pubmed-meshheading:1325913-Deoxyribonuclease I,
pubmed-meshheading:1325913-Gene Expression,
pubmed-meshheading:1325913-HeLa Cells,
pubmed-meshheading:1325913-Humans,
pubmed-meshheading:1325913-Liver Neoplasms,
pubmed-meshheading:1325913-Mice,
pubmed-meshheading:1325913-Mice, Inbred C57BL,
pubmed-meshheading:1325913-Mice, Inbred DBA,
pubmed-meshheading:1325913-Molecular Sequence Data,
pubmed-meshheading:1325913-Nuclear Proteins,
pubmed-meshheading:1325913-Promoter Regions, Genetic,
pubmed-meshheading:1325913-Transcription, Genetic,
pubmed-meshheading:1325913-Transfection,
pubmed-meshheading:1325913-Tumor Cells, Cultured
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Transiently and stably introduced CCAAT/enhancer-binding-protein genes are constitutively expressed in cultured cells.
|
|
pubmed:affiliation |
Karolinska Institute, Center for Biotechnology, Novum, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
