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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1992-10-13
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pubmed:abstractText |
Two isozymes of cGMP-dependent protein kinase (cGMP kinase) have been identified. Polyclonal antibodies were developed which recognize both isozymes or specifically the I alpha and I beta isoform. The specificity of these antibodies was verified by using the recombinant or purified I alpha and I beta isozymes. The antibodies cross-reacted with the purified isozymes of cGMP kinase from bovine tracheal smooth muscle. The tissue concentration of cGMP kinase was determined by ELISA. High concentrations (greater than 10 pmol/g wet tissue) were present in bovine lung, rumen, trachea, aorta, uterus and stomach. The tissue distribution of the isozymes I alpha and I beta was investigated by immunoblots using crude extracts of the different tissues. The I beta-specific antibody yielded strong signals with extracts of trachea, aorta, stomach and uterus, whereas heart, cerebellum and lung apparently contain mainly the I alpha isozyme.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
208
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
467-73
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1325910-Amino Acid Sequence,
pubmed-meshheading:1325910-Animals,
pubmed-meshheading:1325910-Antibodies,
pubmed-meshheading:1325910-Antibody Specificity,
pubmed-meshheading:1325910-CHO Cells,
pubmed-meshheading:1325910-Cattle,
pubmed-meshheading:1325910-Cricetinae,
pubmed-meshheading:1325910-Cyclic GMP,
pubmed-meshheading:1325910-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1325910-Female,
pubmed-meshheading:1325910-Immunoblotting,
pubmed-meshheading:1325910-Isoenzymes,
pubmed-meshheading:1325910-Molecular Sequence Data,
pubmed-meshheading:1325910-Organ Specificity,
pubmed-meshheading:1325910-Protein Kinases,
pubmed-meshheading:1325910-Tissue Distribution
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pubmed:year |
1992
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pubmed:articleTitle |
Detection of cGMP dependent protein kinase isozymes by specific antibodies.
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pubmed:affiliation |
Institut für Pharmakologie und Toxikologie, Technische Universität München, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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