Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1992-10-7
|
| pubmed:abstractText |
The effects of dopamine in vitro were investigated on the binding sites for cholecystokinin-8 (sulphated, CCK-8) and neurotensin in membrane preparations of the caudate-putamen and nucleus accumbens of post-mortem human brains. Dopamine reduced the IC50 value of competition curves with CCK-8 for [125I]CCK-8 binding in membranes from the caudate-putamen, but not the nucleus accumbens, with a maximal decrease of -25 +/- 9% at 300 nM of dopamine. This decrease could be antagonized by 100 nM of SCH 23390 or 100 nM of raclopride. Kinetic analysis of [125I]CCK-8 binding showed a decrease in the first order dissociation rate constant and in the kinetic Kd (-22 +/- 6% and -24 +/- 6%, respectively) at 300 nM of dopamine, without any significant effect on the apparent or actual association rate constant. Competition curves with neurotensin versus [125I]neurotensin were not affected by dopamine (10-1000 nM) in membranes from the caudate-putamen or the nucleus accumbens. These results suggest that dopamine, by synergistic stimulation of both D1 and D2 receptors, selectively increases the affinity of CCK-8 receptors in the human caudate-putamen, by a selective inhibition of ligand dissociation. This increase may reflect a positive feed-back mechanism, further enhancing the modulatory effects of CCK-8 on dopamine neurotransmission.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cholecystokinin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotransmitter
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-8993
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
3
|
| pubmed:volume |
584
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
157-62
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1325243-Adult,
pubmed-meshheading:1325243-Aged,
pubmed-meshheading:1325243-Aging,
pubmed-meshheading:1325243-Binding, Competitive,
pubmed-meshheading:1325243-Caudate Nucleus,
pubmed-meshheading:1325243-Dopamine Antagonists,
pubmed-meshheading:1325243-Female,
pubmed-meshheading:1325243-Humans,
pubmed-meshheading:1325243-Kinetics,
pubmed-meshheading:1325243-Male,
pubmed-meshheading:1325243-Membranes,
pubmed-meshheading:1325243-Middle Aged,
pubmed-meshheading:1325243-Nucleus Accumbens,
pubmed-meshheading:1325243-Putamen,
pubmed-meshheading:1325243-Receptors, Cholecystokinin,
pubmed-meshheading:1325243-Receptors, Dopamine,
pubmed-meshheading:1325243-Receptors, Dopamine D1,
pubmed-meshheading:1325243-Receptors, Dopamine D2,
pubmed-meshheading:1325243-Receptors, Neurotensin,
pubmed-meshheading:1325243-Receptors, Neurotransmitter
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Coactivation of dopamine D1 and D2 receptors increases the affinity of cholecystokinin-8 receptors in membranes from post-mortem human caudate-putamen.
|
| pubmed:affiliation |
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|