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rdf:type |
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lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0033684,
umls-concept:C0206558,
umls-concept:C0242210,
umls-concept:C0439659,
umls-concept:C0678594,
umls-concept:C0679729,
umls-concept:C1167622,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C2369738
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pubmed:issue |
24
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pubmed:dateCreated |
1992-9-25
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pubmed:abstractText |
The origin binding protein (OBP) of herpes simplex virus type 1 is required to activate a viral origin of replication in vivo. We have used intact OBP as well as a truncated form of the protein expressed in Escherichia coli to investigate the protein-protein interactions, as well as the protein-DNA interactions involved in the formation of a nucleoprotein complex at a viral origin of replication (oriS) in vitro. The salient findings demonstrate that the N-terminal part of OBP is required for the cooperative binding of OBP to three sites (boxes I, II, and III) within oriS. A detailed model for the interaction of OBP with the viral origins of replication oriS and oriL is presented.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17424-9
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pubmed:dateRevised |
2007-10-11
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pubmed:meshHeading |
pubmed-meshheading:1324937-Amino Acid Sequence,
pubmed-meshheading:1324937-Animals,
pubmed-meshheading:1324937-Base Sequence,
pubmed-meshheading:1324937-Binding Sites,
pubmed-meshheading:1324937-Cattle,
pubmed-meshheading:1324937-Chromatography, Gel,
pubmed-meshheading:1324937-Cloning, Molecular,
pubmed-meshheading:1324937-DNA,
pubmed-meshheading:1324937-DNA, Viral,
pubmed-meshheading:1324937-DNA Replication,
pubmed-meshheading:1324937-DNA-Binding Proteins,
pubmed-meshheading:1324937-Deoxyribonuclease I,
pubmed-meshheading:1324937-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1324937-Escherichia coli,
pubmed-meshheading:1324937-Genes, Viral,
pubmed-meshheading:1324937-Models, Structural,
pubmed-meshheading:1324937-Molecular Sequence Data,
pubmed-meshheading:1324937-Oligodeoxyribonucleotides,
pubmed-meshheading:1324937-Protein Conformation,
pubmed-meshheading:1324937-Simplexvirus,
pubmed-meshheading:1324937-Viral Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
Structural elements required for the cooperative binding of the herpes simplex virus origin binding protein to oriS reside in the N-terminal part of the protein.
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pubmed:affiliation |
Department of Medical Biochemistry, University of Göteborg, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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