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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1992-9-29
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pubmed:databankReference |
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pubmed:abstractText |
To investigate the role of K-ras mutations in canine non-small cell lung cancer, we first determined the nucleotide sequence of the normal canine K-ras gene and then examined 21 canine lung tumors for activating K-ras mutations. Canine K-ras was analyzed by direct sequencing of polymerase chain reaction products generated with oligonucleotide primers derived from the human K-ras sequence. Four nucleotide differences were found between the canine and human K-ras sequence from position 5 to 211. The deduced amino acid sequence of the canine gene was identical to that of the human. Activated K-ras alleles were detected in 5 of the 21 canine lung tumors examined. The activating lesions were point mutations, predominantly in codon 12. Of the 14 adenocarcinomas examined, 2 (14%) had K-ras mutations. Two of 5 (40%) adenosquamous carcinomas and the only large cell carcinoma also contained activated alleles. The overall frequency of K-ras point mutation in non-small cell lung cancer (25%) is similar to that reported in human non-small cell lung cancer. We conclude that K-ras activation by point mutation is associated with, but not necessary for, non-small cell lung cancer development in the dog.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0008-5472
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
52
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
4724-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1324792-Animals,
pubmed-meshheading:1324792-Base Sequence,
pubmed-meshheading:1324792-Carcinoma,
pubmed-meshheading:1324792-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:1324792-DNA, Neoplasm,
pubmed-meshheading:1324792-Dogs,
pubmed-meshheading:1324792-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:1324792-Genes, ras,
pubmed-meshheading:1324792-Lung Neoplasms,
pubmed-meshheading:1324792-Molecular Sequence Data,
pubmed-meshheading:1324792-Mutation,
pubmed-meshheading:1324792-Oligodeoxyribonucleotides,
pubmed-meshheading:1324792-Polymerase Chain Reaction,
pubmed-meshheading:1324792-Proto-Oncogene Proteins p21(ras),
pubmed-meshheading:1324792-RNA, Messenger,
pubmed-meshheading:1324792-RNA, Neoplasm
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pubmed:year |
1992
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pubmed:articleTitle |
K-ras activation in non-small cell lung cancer in the dog.
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pubmed:affiliation |
School of Veterinary Medicine, University of California, Davis 95616.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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