Linked Life Data

1324199

Source:http://linkedlifedata.com/resource/pubmed/id/1324199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-9-21
pubmed:abstractText
In Escherichia coli DNA topoisomerase I deletion mutant DM800, transcription of the tetracycline-resistance gene (tet) in the pBR322 genome is thought to create and maintain two domains of positive supercoils ahead, and negative supercoils behind, the transcription complex. To assess the actual intracellular state of twin-supercoiled domains, par sequence (365 bp) of plasmid pSC101, which shows a high affinity for DNA gyrase, was inserted into the EcoRI site upstream, or the AvaI site downstream, of the tet gene on the pBR322 genome. Analysis of the oxolinic acid-induced sites of cleavage by gyrase in DM800 revealed that the pBR322 derivatives are highly preferentially cleaved at the par sequence of the EcoRI site as well as the AvaI site and efficiently linearized when compared with pBR322. Assessment of the state of negative supercoiling of the pBR322 derivatives isolated suggested that the DNA (containing the AvaI site) ahead of the tet transcripts, is not so positively supercoiled and preferential interaction of gyrase with the EcoRI-par sequence does not result in removing negative superhelical turns so effectively as DNA topoisomerase I does on pBR322 DNA in the isogenic wild-type cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
72
pubmed:geneSymbol
par, tet
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
A pSC101-par sequence-mediated study on the intracellular state of supercoiling of the pBR322 genome in Escherichia coli DNA topoisomerase I deletion mutant.
pubmed:affiliation
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't