|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0012940,
umls-concept:C0013879,
umls-concept:C0018873,
umls-concept:C0028581,
umls-concept:C0185023,
umls-concept:C0205314,
umls-concept:C0237497,
umls-concept:C0679622,
umls-concept:C1179920,
umls-concept:C1314972,
umls-concept:C1415649,
umls-concept:C1510827,
umls-concept:C1880022,
umls-concept:C1947904,
umls-concept:C1999228,
umls-concept:C2825781
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1992-9-22
|
|
pubmed:abstractText |
We identified four proteins in nuclear extracts from HeLa cells which specifically bind to a scaffold attachment region (SAR) element from the human genome. Of these four proteins, SAF-A (scaffold attachment factor A), shows the highest affinity for several homologous and heterologous SAR elements from vertebrate cells. SAF-A is an abundant nuclear protein and a constituent of the nuclear matrix and scaffold. The homogeneously purified protein is a novel double stranded DNA binding protein with an apparent molecular weight of 120 kDa. SAF-A binds at multiple sites to the human SAR element; competition studies with synthetic polynucleotides indicate that these sites most probably reside in the multitude of A/T-stretches which are distributed throughout this element. In addition we show by electron microscopy that the protein forms large aggregates and mediates the formation of looped DNA structures.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1553545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1652367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1711534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1795015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1846084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1851751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1892652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1904543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1946399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-1991107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2040622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2068100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2118999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2155393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2167839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2233749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2357967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2544867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2546156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2556260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2614834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2655930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2656680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2686987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2797152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2840282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2844236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2985626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2987966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-2990913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3002631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3015418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3226138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3315231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3418709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3430625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-3763392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-4038503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-4214419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-6091913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-6175245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-6269093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1324173-7213377
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
11
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3431-40
|
|
pubmed:dateRevised |
2010-9-7
|
|
pubmed:meshHeading |
pubmed-meshheading:1324173-Animals,
pubmed-meshheading:1324173-Chromosome Mapping,
pubmed-meshheading:1324173-DNA,
pubmed-meshheading:1324173-DNA Topoisomerases, Type I,
pubmed-meshheading:1324173-DNA-Binding Proteins,
pubmed-meshheading:1324173-HeLa Cells,
pubmed-meshheading:1324173-Humans,
pubmed-meshheading:1324173-Nuclear Matrix,
pubmed-meshheading:1324173-Nuclear Proteins,
pubmed-meshheading:1324173-Protein Binding,
pubmed-meshheading:1324173-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1324173-Species Specificity
|
|
pubmed:year |
1992
|
