Linked Life Data

1323058

Source:http://linkedlifedata.com/resource/pubmed/id/1323058

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-9-4
pubmed:abstractText
T4-binding globulin (TBG) shares a high degree of homology with two serpin antiproteases, alpha 1-antichymotrypsin (ACT) and alpha 1-antitrypsin (AT), whose synthesis is increased during the acute phase phenomenon, which accompanies trauma, infections, and neoplasms. Interleukin-6 (IL-6) is believed to be the main effector of the acute phase response. When evaluated in human hepatoblastoma-derived (Hep G2) cells exposed to different doses of the recombinant human cytokine for variable time intervals, IL-6 caused a dose- and time-dependent decrease in the secretion of [35S]methionine-labeled TBG, transthyretin (TTR), and albumin. The secretion of ACT and AT was increased. These changes were not due to alterations in the secretory process, since the kinetics of secretion of newly synthesized proteins were not modified. IL-6 did, however, cause a decrease in the steady state levels of mRNA for TTR, TBG, and albumin and an increase in ACT and AT mRNAs. In addition, nuclear run-off assay demonstrated a decrease in the transcription of TTR, TBG, and albumin genes and an increased transcription of the ACT gene. Quantitation of the results showed that changes in the secretion of proteins, in steady state mRNA levels, and in gene transcription were superimposable for each protein, indicating that IL-6 exerts its effect on thyroid hormone-binding proteins mostly at the transcriptional level and that TTR is the thyroid hormone-binding protein showing the most pronounced negative regulation by IL-6. The opposite effect of IL-6 on TBG and the antiproteases, despite their structural homology, underscores gene divergence among these proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:geneSymbol
TBG, TTR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
935-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1323058-Acute-Phase Reaction, pubmed-meshheading:1323058-Albumins, pubmed-meshheading:1323058-Carcinoma, Hepatocellular, pubmed-meshheading:1323058-Enzyme Induction, pubmed-meshheading:1323058-Gene Expression Regulation, Neoplastic, pubmed-meshheading:1323058-Humans, pubmed-meshheading:1323058-Interleukin-6, pubmed-meshheading:1323058-Liver Neoplasms, pubmed-meshheading:1323058-Neoplasm Proteins, pubmed-meshheading:1323058-Prealbumin, pubmed-meshheading:1323058-RNA, Messenger, pubmed-meshheading:1323058-RNA, Neoplasm, pubmed-meshheading:1323058-Recombinant Proteins, pubmed-meshheading:1323058-Secretory Rate, pubmed-meshheading:1323058-Sequence Homology, Nucleic Acid, pubmed-meshheading:1323058-Thyroxine-Binding Proteins, pubmed-meshheading:1323058-Transcription, Genetic, pubmed-meshheading:1323058-Tumor Cells, Cultured, pubmed-meshheading:1323058-alpha 1-Antichymotrypsin, pubmed-meshheading:1323058-alpha 1-Antitrypsin
pubmed:year
1992
pubmed:articleTitle
Effects of interleukin-6 on the expression of thyroid hormone-binding protein genes in cultured human hepatoblastoma-derived (Hep G2) cells.
pubmed:affiliation
Genetics and Biochemistry Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article, Comparative Study