Linked Life Data

1322917

Source:http://linkedlifedata.com/resource/pubmed/id/1322917

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-9-10
pubmed:abstractText
The effects of sodium butyrate on simian virus 40 early gene expression were determined in SV40-transformed human embryonic lung fibroblasts (SVWI-38). Northern blot analysis and nuclear run-off transcription studies revealed that treatment of cells with millimolar concentrations of sodium butyrate (2.5 to 10 mM) resulted in increased levels of SV40 early gene transcripts, with a concomitant increase in their corresponding proteins (large-T and small-t antigens). Although sodium butyrate treatment enhanced the expression of the early genes, it was associated with a reduction in cell growth and total protein synthesis, as measured by cell number and incorporation of 3H-leucine into macromolecules, respectively. Immunoprecipitation of 35S-labelled cellular proteins with anti-p53 and anti-T antibodies revealed that the level of the cellular protein, p53, declined markedly in the presence of sodium butyrate. Furthermore, in control cells only 30% of the p53 was complexed with large-T antigen, whereas in butyrate-treated cells all the p53 was complexed with large-T antigen. The increased early gene expression was not due to altered methylation patterns, gene amplification, or rearrangement of the integrated SV40 genome. Sodium butyrate treatment did, however, result in the appearance of a new nuclear protein which bound specifically to a SV40 promoter fragment containing large-T antigen binding sites I and II.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
74-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1322917-Antigens, Polyomavirus Transforming, pubmed-meshheading:1322917-Blotting, Southern, pubmed-meshheading:1322917-Butyric Acid, pubmed-meshheading:1322917-Butyric Acids, pubmed-meshheading:1322917-Cell Transformation, Viral, pubmed-meshheading:1322917-Cells, Cultured, pubmed-meshheading:1322917-DNA, Viral, pubmed-meshheading:1322917-DNA-Binding Proteins, pubmed-meshheading:1322917-Fibroblasts, pubmed-meshheading:1322917-Gene Expression Regulation, Viral, pubmed-meshheading:1322917-Humans, pubmed-meshheading:1322917-Methylation, pubmed-meshheading:1322917-Precipitin Tests, pubmed-meshheading:1322917-RNA, Messenger, pubmed-meshheading:1322917-RNA, Viral, pubmed-meshheading:1322917-Simian virus 40, pubmed-meshheading:1322917-Transcription, Genetic, pubmed-meshheading:1322917-Tumor Suppressor Protein p53
pubmed:year
1992
pubmed:articleTitle
Elevation of large-T antigen production by sodium butyrate treatment of SV40-transformed WI-38 fibroblasts.
pubmed:affiliation
MRC/UCT Research Unit for the Cell Biology of Atherosclerosis, Department of Medical Biochemistry, University of Cape Town Medical School, Observatory, South Africa.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't