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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-8-14
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pubmed:abstractText |
Elsamicin A is an antitumor antibiotic with fascinating chemical structure and a good candidate for pharmaceutical development. Molecular mechanism of DNA backbone cleavage mediated by Fe(II)-elsamicin A has been examined. Product analysis using DNA sequencing gels and HPLC reveals the production of damaged DNA fragments bearing 3'-/5'-phosphate and 3'-phosphoglycolate termini associated with formation of free base. In addition, hydrazine-trapping experiments indicate that C-4' hydroxylated abasic sites are formed concomitant with DNA degradation by Fe(II)-elsamicin A. The results lead to the conclusion that the hydroxyl radical formed in Fe(II)-elsamicin A plus dithiothreitol system oxidizes the deoxyribose moiety via hydrogen abstraction predominantly at the C-4' carbon of the deoxyribose backbone and ultimately produces strand breakage of DNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoglycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxides,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyl Radical,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Polydeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/elsamicin A,
http://linkedlifedata.com/resource/pubmed/chemical/poly(dC-dG)
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
186
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
580-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1321617-Aminoglycosides,
pubmed-meshheading:1321617-Anti-Bacterial Agents,
pubmed-meshheading:1321617-Antibiotics, Antineoplastic,
pubmed-meshheading:1321617-Base Sequence,
pubmed-meshheading:1321617-Carbohydrate Sequence,
pubmed-meshheading:1321617-DNA,
pubmed-meshheading:1321617-DNA, Bacterial,
pubmed-meshheading:1321617-DNA Damage,
pubmed-meshheading:1321617-Free Radicals,
pubmed-meshheading:1321617-Hydroxides,
pubmed-meshheading:1321617-Hydroxyl Radical,
pubmed-meshheading:1321617-Iron,
pubmed-meshheading:1321617-Kinetics,
pubmed-meshheading:1321617-Molecular Sequence Data,
pubmed-meshheading:1321617-Molecular Structure,
pubmed-meshheading:1321617-Oligodeoxyribonucleotides,
pubmed-meshheading:1321617-Plasmids,
pubmed-meshheading:1321617-Polydeoxyribonucleotides,
pubmed-meshheading:1321617-Restriction Mapping
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pubmed:year |
1992
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pubmed:articleTitle |
Product analyses in DNA strand scission by antitumor antibiotic elsamicin A.
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pubmed:affiliation |
Institute for Chemical Research, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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