|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1977-3-15
|
|
pubmed:abstractText |
1. The intracellular pH of intact single muscle fibres of the giant barnacle was measured directly with a glass micro-electrode following prolonged (2-5 hr) equilibration in one of three solutions: normal Ringer, CO2 Ringer and NH4+ Ringer. 2. The intracellular pH of identically-prepared fibres from the same specimen was measured indirectly from the distribution of DMO following prolonged equilibration in the same solutions. 3. The DMO-pH compared favourably with the electrode-pHi provided DMO-pHi was calculated from the values of the indicator compounds, [14C]DMO and [3H]inulin, obtained by extrapolating the slow uptake phase to time zero. 4. Following prolonged equilibration, the transmembrane H+ ion distribution was found to vary with the membrane potential but not in accordance with a simple Gibbs-Donnan equilibrium. 5. A model which recognizes the existence of two independent net fluxes for H+ across the membrane in developed to explain the results. One of the fluxes represents passive diffusion and the other represents the so called H+-pump. The model predicts the H+-pump rate increases by two orders of magnitude when pHi is reduced from 7-2 to 6-7.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-13564418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-13654506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-14212147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-1523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-239228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-4264604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-4536964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-4743524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-4838803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-4873193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-4889321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-5039276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-5246543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-5651768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-5810605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-5970954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-6026098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/13203-6052742
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0022-3751
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
262
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
533-52
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:13203-Animals,
pubmed-meshheading:13203-Biological Transport, Active,
pubmed-meshheading:13203-Body Fluids,
pubmed-meshheading:13203-Dimethadione,
pubmed-meshheading:13203-Extracellular Space,
pubmed-meshheading:13203-Hydrogen,
pubmed-meshheading:13203-Hydrogen-Ion Concentration,
pubmed-meshheading:13203-Intracellular Fluid,
pubmed-meshheading:13203-Membrane Potentials,
pubmed-meshheading:13203-Muscles,
pubmed-meshheading:13203-Thoracica
|
|
pubmed:year |
1976
|
|
pubmed:articleTitle |
Intracellular pH of single crustacean muscle fibres by the DMO and electrode methods during acid and alkaline conditions.
|
|
pubmed:publicationType |
Journal Article,
In Vitro
|
