Linked Life Data

1320252

Source:http://linkedlifedata.com/resource/pubmed/id/1320252

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-8-6
pubmed:abstractText
NPPB (5-nitro-2-(3-phenylpropylamino)-benzoate), a member of the novel class of Cl- channel blockers related to diphenylamine-2-carboxylate, was studied for its effect on the Ca(++)-activated Cl- current (ICl(Ca)) in frog (Xenopus laevis) oocytes. ICl(Ca) was activated by bath application of 5 mM Ca++ to oocytes that had been Ca++ permeabilized with the Ca++ ionophore A23187. In order to prevent the inactivation of ICl(Ca) that occurs with repetitive applications of Ca++, oocytes were also treated with H-7 (1,5-isoquinolinesulfonyl-1,2 methylpiperazine), an inhibitor of protein kinases. NPPB reversibly blocked both the fast transient (Ifast) and slow (Islow) components of ICl(Ca) with inhibition constants of 22 microM and 68 microM, respectively. NPPB block of ICl(Ca) was voltage dependent and potentiated by depolarization of the oocyte membrane. Our results indicate that NPPB is a potent blocker of the oocyte endogenous ICl(Ca), and may prove useful in the study of exogenously expressed Cl channels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
420
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
227-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
NPPB block of Ca(++)-activated Cl- currents in Xenopus oocytes.
pubmed:affiliation
Section of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't