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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-7-16
|
| pubmed:abstractText |
Immunoreactivity for two derivatives of pro-opiomelanocortin, beta-endorphin and alpha-melanocortin (or corticotropin), was demonstrated, using a conventional immunoperoxidase method, in some of the intramuscular nerves in muscle sections from obese diabetic (ob/ob) mice and homozygous lean (+/+) mice. The endplate regions were visualized in the sections by staining for acetylcholinesterase reaction product. The proportion of muscle endplates with beta-endorphin-immunoreactive motor nerves was approximately 2.5-fold higher in soleus and extensor digitorum longus muscles and approximately 1.5-fold higher in the diaphragm of the obese (ob/ob) mice compared to the normal lean mice. The proportion of muscle endplates with alpha-melanotropin-immunoreactive motor nerves was between 30 and 53% lower, depending on the muscle type, in the ob/ob mice compared to the lean mice. The muscles of ob/ob and lean mice were investigated for the presence of specific binding sites for [125I]beta-endorphin and for [125I]corticotropin, using autoradiography. Some muscle fibres in soleus, extensor digitorum longus and diaphragm in both the ob/ob and the lean mice exhibited specific binding sites for the radioactive ligands. The binding sites were distributed over the entire surface in these muscle fibres. In the ob/ob mice the number of muscle fibres with specific [125I]beta-endorphin binding sites was six-fold higher in soleus and approximately 10-fold higher in extensor digitorum longus and diaphragm, than in the corresponding muscles of the lean mice. In contrast, the number of muscle fibres with specific [125I]corticotropin binding sites was similar in obese (ob/ob) and lean mice.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Corticotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-MSH,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Endorphin
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0306-4522
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
48
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
463-8
|
| pubmed:dateRevised |
2009-9-29
|
| pubmed:meshHeading |
pubmed-meshheading:1318515-Adrenocorticotropic Hormone,
pubmed-meshheading:1318515-Animals,
pubmed-meshheading:1318515-Autoradiography,
pubmed-meshheading:1318515-Binding Sites,
pubmed-meshheading:1318515-Diabetes Mellitus,
pubmed-meshheading:1318515-Diabetes Mellitus, Experimental,
pubmed-meshheading:1318515-Immunoenzyme Techniques,
pubmed-meshheading:1318515-Iodine Radioisotopes,
pubmed-meshheading:1318515-Mice,
pubmed-meshheading:1318515-Mice, Inbred C57BL,
pubmed-meshheading:1318515-Mice, Obese,
pubmed-meshheading:1318515-Motor Endplate,
pubmed-meshheading:1318515-Muscles,
pubmed-meshheading:1318515-Obesity,
pubmed-meshheading:1318515-Receptors, Corticotropin,
pubmed-meshheading:1318515-Receptors, Opioid,
pubmed-meshheading:1318515-Receptors, Pituitary Hormone,
pubmed-meshheading:1318515-Reference Values,
pubmed-meshheading:1318515-alpha-MSH,
pubmed-meshheading:1318515-beta-Endorphin
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Beta-endorphin and corticotropin immunoreactivity and specific binding in the neuromuscular system of obese-diabetic mice.
|
| pubmed:affiliation |
Department of Physiology, Medical School, University of Birmingham, U.K.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|