Linked Life Data

1316440

Source:http://linkedlifedata.com/resource/pubmed/id/1316440

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-6-18
pubmed:abstractText
The solubilized perivitelline layer (PL) of the chicken ovum contains one or more components which behave in a manner analogous to sperm-receptors. Electrophoretic analyses of solubilized PL confirmed the existence of three major glycoproteins having apparent molecular weights of 33,000, 53,500 and greater than 200,000. The role of carbohydrate in sperm receptor activity was evaluated by extensive deglycosylation of the solubilized PL with trifluoromethanesulfonic acid (TFMS). Sperm receptor activity as measured by an in vitro competition assay was extremely sensitive to TFMS. Pretreatment of spermatozoa with solubilized PL exposed only to TFMS buffers inhibited sperm attachment and digestion of intact PL by 81% (9.67 +/- 1.76 sperm/mm2) as compared with the controls (i.e., no pretreatment of spermatozoa with solubilized PL; 51.33 +/- 6.24 sperm/mm2), a value similar to that observed with spermatozoa exposed to untreated solubilized PL (0.17 +/- 0.17 sperm/mm2). In comparison, PL treated with TFMS inhibited sperm attachment and digestion of intact PL by less than 26% (38.33 +/- 5.88 sperm/mm2) as compared with the control. These data indicate that removal of both N- and O-linked oligosaccharides from components of the chicken PL results in elimination of its sperm receptor activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0197-5110
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Carbohydrate involvement in sperm-egg interaction in the chicken.
pubmed:affiliation
Department of Poultry Science, University of Georgia, Athens 30602.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't