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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-6-12
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned and sequenced a cDNA for the rabbit gastric proton-potassium pump (H+/K(+)-ATPase) alpha-subunit. The deduced peptide contains 1035 amino acids (Mr 114,201) and shows 97% sequence identity with the respective rat and hog proteins. A monoclonal antibody 146-14 has been shown previously to react with the extracytoplasmic side of the catalytic H+/K(+)-ATPase subunit and here we show that the epitope is in the region between amino acids 855 and 902 (the numbering of the H+/K(+)-ATPase catalytic subunit throughout the paper refers to the rabbit sequence). The localization of this epitope in conjunction with previously observed trypsin cleavage sites in the C-terminal one third of the enzyme and the hydrophobicity plot of the deduced peptide sequence are evidence for a structural model for the alpha-subunit of the H+/K(+)-ATPase which contains at least ten membrane spanning segments, similar to that deduced for the Ca(2+)-ATPase of sarcoplasmic reticulum.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
1131
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
69-77
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1316171-Adenosine Triphosphatases,
pubmed-meshheading:1316171-Amino Acid Sequence,
pubmed-meshheading:1316171-Animals,
pubmed-meshheading:1316171-Base Sequence,
pubmed-meshheading:1316171-Binding Sites,
pubmed-meshheading:1316171-Cloning, Molecular,
pubmed-meshheading:1316171-DNA,
pubmed-meshheading:1316171-DNA Probes,
pubmed-meshheading:1316171-Gastric Mucosa,
pubmed-meshheading:1316171-Gene Library,
pubmed-meshheading:1316171-H(+)-K(+)-Exchanging ATPase,
pubmed-meshheading:1316171-Molecular Sequence Data,
pubmed-meshheading:1316171-Peptide Fragments,
pubmed-meshheading:1316171-Polymerase Chain Reaction,
pubmed-meshheading:1316171-Rabbits,
pubmed-meshheading:1316171-Rats,
pubmed-meshheading:1316171-Swine,
pubmed-meshheading:1316171-Trypsin
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pubmed:year |
1992
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pubmed:articleTitle |
cDNA cloning and membrane topology of the rabbit gastric H+/K(+)-ATPase alpha-subunit.
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pubmed:affiliation |
Department of Physiology, School of Medicine, UCLA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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