Linked Life Data

1315934

Source:http://linkedlifedata.com/resource/pubmed/id/1315934

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-6-9
pubmed:abstractText
Type-1-like cortical mouse astrocytes were studied in homogeneous cultures. Membrane input resistance and membrane potential were measured during drug-induced inhibition of glycolysis (sodium fluoride), mitochondrial respiration (antimycin-a) and Na+/K+ pump activity (ouabain). It was found that the electrical properties of the astrocytes recovered after a 60 min period with inhibited glycolysis or mitochondrial respiration, exhibiting only small reversible depolarizations. A 60 min period of high K(+)-induced depolarization, of cell swelling or of Na+/K+ pump inhibition does not lead to irreversible changes. Total block of energy metabolism, however, causes (1) a large depolarization, which is mainly mediated by external calcium, and (2) a 10-fold increase in input resistance, suggestive of an uncoupling of gap junctions. After an exposure period ranging between 45 and 60 min these conditions lead to irreversible damage. This damage appears to be independent of extracellular calcium and the degree of depolarization and to be specifically mediated by events occurring after the 60-min period of inhibited cell metabolism, that is during the recovery period.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
203-11
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Metabolic inhibition and electrical properties of type-1-like cortical astrocytes.
pubmed:affiliation
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't