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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-5-28
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pubmed:abstractText |
We have developed a sensitive genetic assay to analyze DNA sequences and regulatory elements required for immunoglobulin heavy chain isotype switch recombination. Recombination substrates containing mu and gamma 3 chain switch (S)-region sequences, S mu and S gamma 3, are transiently introduced into primary murine B cells cultured with lipopolysaccharide to induce isotype switching. Recombination involving S-region sequences deletes a conditionally lethal marker, the leftward promoter of phage lambda (lambda PL), enabling recovered plasmids to transform Escherichia coli. In substrates carrying S mu-lambda PL-S gamma 3, about 2% of replicated molecules undergo deletion of lambda PL during transfection; insertion of either the immunoglobulin heavy chain promoter and enhancer sequences or cytomegalovirus IE1 promoter region upstream of S mu increases recombination 10-fold or more to 25% of replicated molecules. Guanosine-rich S-region sequences are essential for efficient recombination of these substrates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-1701258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-1900920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-1986861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2000372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2106137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2111465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2114219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2157139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2165864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2174540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2188677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2208275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2320109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2339132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2364432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2459714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2495537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2505932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2549853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2578321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2590943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2601741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2645056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2694945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2777075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2785916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2822395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2831538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2834063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2841949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-2987353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3007121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3023199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3030563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3086846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3132612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3144437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3317825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3393228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3428598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3495343,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-3690664,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1315054-6315407
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4154-8
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1315054-Animals,
pubmed-meshheading:1315054-B-Lymphocytes,
pubmed-meshheading:1315054-Cells, Cultured,
pubmed-meshheading:1315054-Cytomegalovirus,
pubmed-meshheading:1315054-Enhancer Elements, Genetic,
pubmed-meshheading:1315054-Genes, Immunoglobulin,
pubmed-meshheading:1315054-Genes, Switch,
pubmed-meshheading:1315054-Mice,
pubmed-meshheading:1315054-Mice, Inbred BALB C,
pubmed-meshheading:1315054-Plasmids,
pubmed-meshheading:1315054-Promoter Regions, Genetic,
pubmed-meshheading:1315054-Recombination, Genetic,
pubmed-meshheading:1315054-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1315054-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Transcriptional regulatory elements stimulate recombination in extrachromosomal substrates carrying immunoglobulin switch-region sequences.
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pubmed:affiliation |
Department of Molecular Biophysics and Biochemistry, Yale Medical School, New Haven, CT 06510.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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