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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1992-5-28
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pubmed:abstractText |
The control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2; EC 2.7.1.105/3.1.3.46) gene expression during liver regeneration was studied. The level of PFK-2/FBPase-2 mRNA decreased to about 5% of the control value 6 hr after partial hepatectomy. Thereafter the mRNA increased to a maximum at 48 hr and returned to normal levels by 96 hr. In sham-operated animals, only a small increase was observed during the first 4 hr. The mRNA was recognized by a 299-base-pair liver-specific cDNA probe but not by a muscle-specific probe. The time course of mRNA modulation was well correlated with PFK-2/FBPase-2 activity and with the amount of bifunctional enzyme protein determined by immunoblotting with an antibody raised against the N-terminal decapeptide of liver PFK-2/FBPase-2. No alteration in the degradation rate of PFK-2/FBPase-2 mRNA was noted after partial hepatectomy. The modulation of PFK-2/FBPase-2 gene expression during liver regeneration involved changes in the transcription rate. The rate decreased by 50% at 6 hr after liver resection. The rate increased thereafter to a maximum at 72 hr and then returned to control values by 96 hr. The transcription rate of albumin did not change, whereas that of phosphoenolpyruvate carboxykinase increased 12-fold at 6 hr. These results show that PFK-2/FBPase-2 gene transcription is specifically regulated and that this regulation is in part responsible for the alterations in hepatic metabolism seen in regenerating liver.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-1201045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-158758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-1694072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-1846360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-1846662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2123877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2162755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2173548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2191945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2195041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-220134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2404819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2407480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2540002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2540168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2547373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2549541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2555217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2557826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2642989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2779551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2822019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2824312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2826245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-2848802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-3028056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-3052289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-3841792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-4772620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-488112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-5572901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6030054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6096365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6187744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6266278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6291025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6297885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6304730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6312964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6353247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-6772444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1315037-979645
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3746-50
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1315037-Animals,
pubmed-meshheading:1315037-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:1315037-Hepatectomy,
pubmed-meshheading:1315037-Liver,
pubmed-meshheading:1315037-Liver Regeneration,
pubmed-meshheading:1315037-Multienzyme Complexes,
pubmed-meshheading:1315037-Phosphoenolpyruvate Carboxykinase (GTP),
pubmed-meshheading:1315037-Phosphofructokinase-2,
pubmed-meshheading:1315037-Phosphoric Monoester Hydrolases,
pubmed-meshheading:1315037-Phosphotransferases,
pubmed-meshheading:1315037-RNA, Messenger,
pubmed-meshheading:1315037-Rats,
pubmed-meshheading:1315037-Rats, Inbred Strains,
pubmed-meshheading:1315037-Time Factors,
pubmed-meshheading:1315037-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Transcriptional and posttranscriptional regulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase during liver regeneration.
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pubmed:affiliation |
Departament de Ciències Fisiològiques Humanes i de la Nutrició, Universitat de Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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