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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-5-26
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59212,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59213,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59214,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59215,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59216,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59217,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59218,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59219,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59220,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59221,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59222,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59223,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59224,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59225,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59228,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59230,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59231,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59232,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59233,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59234,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59235,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X59236
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pubmed:abstractText |
LINE-1 (L1) is a mammalian family of highly repeated DNA sequences that are members of a class of transposable elements whose movement involves an RNA intermediate. Both structural and evolutionary data indicate that the L1 family consists of a small number of active transposable elements interspersed with a large number of L1 pseudogenes. In the mouse, the longest, characterized L1 sequences span about 7000 base-pairs and contain two long open reading frames. Two subfamilies of mouse L1 elements, A and F, have been defined on the basis of the type of putative transcriptional regulatory sequence found at the 5' end. In order to identify a transcribed subset of L1 elements in mouse F9 teratocarcinoma cells, we have examined the strand-specificity of L1 transcription by Northern analysis and compared the open reading frame-1 sequences of ten A-type cDNAs with fifteen genomic A-type L1 elements. Transcripts containing A-type sequence are far more abundant than those containing F-type sequence. Although the majority of L1 RNA in F9 cells appears to be transcribed non-specifically from both strands, our results provide evidence for a subpopulation of variable length, strand-specific transcripts arising from A-type transcriptional regulatory sequences. F9 cell cDNA sequences, which share greater than 99.5% sequence identity with one another, represent a homogeneous subset of the genomic L1 population. Examination of genomic mouse L1 sequences reveals three types of length polymorphism in a defined segment of the first open reading frame. Phylogenetic analysis shows a correlation between the type of length polymorphism in the first open reading frame and the relative age of an individual A-type genomic L1 element. Comparison of the cDNA and genomic sequences indicates that the youngest subgroup of A-type L1 elements is preferentially transcribed in F9 cells. This subgroup may be currently dominating the L1 dispersal process in mice.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-2836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
224
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
559-74
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1314898-Amino Acid Sequence,
pubmed-meshheading:1314898-Animals,
pubmed-meshheading:1314898-Base Sequence,
pubmed-meshheading:1314898-Cloning, Molecular,
pubmed-meshheading:1314898-DNA, Neoplasm,
pubmed-meshheading:1314898-DNA Probes,
pubmed-meshheading:1314898-DNA Transposable Elements,
pubmed-meshheading:1314898-Mice,
pubmed-meshheading:1314898-Molecular Sequence Data,
pubmed-meshheading:1314898-Multigene Family,
pubmed-meshheading:1314898-Phylogeny,
pubmed-meshheading:1314898-Polymorphism, Genetic,
pubmed-meshheading:1314898-RNA, Neoplasm,
pubmed-meshheading:1314898-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1314898-Teratoma,
pubmed-meshheading:1314898-Transcription, Genetic,
pubmed-meshheading:1314898-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Strand-specific LINE-1 transcription in mouse F9 cells originates from the youngest phylogenetic subgroup of LINE-1 elements.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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