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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-5-20
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pubmed:abstractText |
Thyroliberin E-H-P-NH2 (TRH) is a small neuropeptide pGlu-His-Pro-NH2 widely distributed in neural sites. The aim of this work was to obtain an antibody molecule with the nearest properties to that of TRH-receptor in GH3 cells. Different TRH-protein conjugates were prepared and utilized to induce monoclonal antibodies in mice. Several monoclonal antibodies were obtained using E-H-P-NH2 (TRH) coupled either to the histidyl residue (immunogen I) or to the prolyl residue (immunogen II). Antibodies generated using immunogen I and immunogen II were characterized in a radioimmunoassay system and an enzyme immunoassay system respectively. Their selectivities regarding a series of TRH related peptides were compared to those of rabbit polyclonal antibodies using three differently labelled TRH (tritiated-TRH, mono-iodinated-TRH and TRH-OH-acetyl-cholinesterase) as tracers and to prolactin secreting cells TRH receptors using 3H-TRH. Whatever the immunogen, the stereospecificity of monoclonal antibodies tested were found more different from TRH receptor characteristics than rabbit polyclonal antibodies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0161-5890
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
463-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1314325-Amino Acid Sequence,
pubmed-meshheading:1314325-Animals,
pubmed-meshheading:1314325-Antibodies, Monoclonal,
pubmed-meshheading:1314325-Antibody Specificity,
pubmed-meshheading:1314325-Antigen-Antibody Reactions,
pubmed-meshheading:1314325-Immunoenzyme Techniques,
pubmed-meshheading:1314325-Immunoglobulin Isotypes,
pubmed-meshheading:1314325-Mice,
pubmed-meshheading:1314325-Mice, Inbred BALB C,
pubmed-meshheading:1314325-Mice, Inbred NZB,
pubmed-meshheading:1314325-Molecular Sequence Data,
pubmed-meshheading:1314325-Pituitary Gland,
pubmed-meshheading:1314325-Rabbits,
pubmed-meshheading:1314325-Radioimmunoassay,
pubmed-meshheading:1314325-Rats,
pubmed-meshheading:1314325-Receptors, Neurotransmitter,
pubmed-meshheading:1314325-Receptors, Thyrotropin-Releasing Hormone,
pubmed-meshheading:1314325-Thyrotropin-Releasing Hormone,
pubmed-meshheading:1314325-Time Factors,
pubmed-meshheading:1314325-Vaccination
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pubmed:year |
1992
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pubmed:articleTitle |
Properties of monoclonal antibodies to thyroliberin (TRH) induced by different immunogens: comparison with pituitary TRH receptor.
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pubmed:affiliation |
Département d'Immunologie, Institut Pasteur (CNRS UA 359, Paris, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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