Linked Life Data

1313948

Source:http://linkedlifedata.com/resource/pubmed/id/1313948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6369
pubmed:dateCreated
1992-5-14
pubmed:abstractText
Translocation of proteins across the endoplasmic reticulum (ER) membrane represents the first step in the eukaryotic secretory pathway. In mammalian cells, the targeting of secretory and membrane protein precursors to the ER is mediated by signal recognition particle (SRP), a cytosolic ribonucleoprotein complex comprising a molecule of 7SL RNA and six polypeptide subunits (relative molecular masses 9, 14, 19, 54, 68 and 72K). In Saccharomyces cerevisiae, a homologue of the 54K subunit (SRP54) co-purifies with a small cytoplasmic RNA, scR1 (refs 4, 5). Genetic data indicate that SRP54 and scR1 are involved in translocation in vivo, suggesting the existence of an SRP-like activity in yeast. Whether this activity requires additional components similar to those found in mammalian SRP is not known. We have recently reported a genetic selection that led to the isolation of a yeast mutant, sec65-1, which is conditionally defective in the insertion of integral membrane proteins into the ER. Here we report the cloning and sequencing of the SEC65 gene, which encodes a 31.2K protein with significant sequence similarity to the 19K subunit of human SRP (SRP19). We also report the cloning of a multicopy suppressor of sec65-1, and its identification as the previously defined SRP54 gene, providing genetic evidence for an interaction between these gene products in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
356
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
534-7
pubmed:dateRevised
2009-7-28
pubmed:meshHeading
pubmed-meshheading:1313948-Amino Acid Sequence, pubmed-meshheading:1313948-Base Sequence, pubmed-meshheading:1313948-Blotting, Western, pubmed-meshheading:1313948-Cell Division, pubmed-meshheading:1313948-Cloning, Molecular, pubmed-meshheading:1313948-Fungal Proteins, pubmed-meshheading:1313948-Gene Expression Regulation, pubmed-meshheading:1313948-Genes, Fungal, pubmed-meshheading:1313948-Genes, Suppressor, pubmed-meshheading:1313948-Humans, pubmed-meshheading:1313948-Molecular Sequence Data, pubmed-meshheading:1313948-Plasmids, pubmed-meshheading:1313948-Ribonucleoproteins, pubmed-meshheading:1313948-Saccharomyces cerevisiae, pubmed-meshheading:1313948-Saccharomyces cerevisiae Proteins, pubmed-meshheading:1313948-Sequence Homology, Nucleic Acid, pubmed-meshheading:1313948-Signal Recognition Particle
pubmed:year
1992
pubmed:articleTitle
The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Medical School, University of Manchester, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't