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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1992-5-14
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pubmed:abstractText |
Human foamy virus (HFV) encodes the transcriptional transactivator bel1. The bel1 protein transactivates HFV long terminal repeat (LTR)-directed gene expression by recognizing a region in U3. It also transactivates human immunodeficiency virus type 1 (HIV-1) LTR-directed gene expression in transient transfection assays. To identify the specific region in HIV-1 LTR responsible for bel1 action, we examined the effect of bel1 on chloramphenicol acetyltransferase (CAT) gene expression in transfected cells with a series of mutant HIV-1 LTR/CAT plasmids. The region between -158 and -118 from the transcription initiation site, immediately upstream of the core enhancer element, was identified as responsible for the transactivation by bel1. In addition, bel1 transactivated a heterologous promoter when this region was positioned upstream of it in the sense and antisense orientations. Optimal transactivation of the HIV-1 LTR by bel1 did not require an intact TAR sequence, suggesting that the binding of tat to the TAR sequence is not a prerequisite for bel1 function in HIV-1 LTR-directed gene expression. In the region of the HIV-1 LTR that is necessary for the bel1-mediated transactivation, we have found a sequence which is conserved between HIV-1 and HFV. Our results suggest that the bel1 action on HIV-1 seems to be mediated by a specific DNA sequence which is shared by both the HIV-1 LTR and HFV LTR.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313928-1690391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313928-1846970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313928-1850032,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1313928-2190099,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1313928-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3236-40
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1313928-Base Sequence,
pubmed-meshheading:1313928-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1313928-DNA Mutational Analysis,
pubmed-meshheading:1313928-DNA-Binding Proteins,
pubmed-meshheading:1313928-HIV Long Terminal Repeat,
pubmed-meshheading:1313928-Humans,
pubmed-meshheading:1313928-Molecular Sequence Data,
pubmed-meshheading:1313928-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1313928-Retroviridae Proteins,
pubmed-meshheading:1313928-Simplexvirus,
pubmed-meshheading:1313928-Spumavirus,
pubmed-meshheading:1313928-Trans-Activators,
pubmed-meshheading:1313928-Transcriptional Activation,
pubmed-meshheading:1313928-Viral Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
Transactivation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by the human foamy virus bel1 protein requires a specific DNA sequence.
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pubmed:affiliation |
Department of Life Science, Pohang Institute of Science and Technology, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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