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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1992-5-14
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pubmed:abstractText |
Avian myelocytomatosis virus MC29 induces a wide variety of neoplastic diseases in infected birds and transforms cells of the macrophage lineage as well as fibroblasts and epithelial cells. A biological and biochemical analysis, carried out on a series of in-frame insertion and deletion mutations within the gag-myc gene of MC29, revealed several mutations within the 5' portion of the v-myc gene that encode proteins either completely defective for transformation or compromised in their ability to transform chicken embryo fibroblasts but not macrophages. Mutations within the 3' end of the v-myc gene which disrupt sequences encoding the basic/helix-loop-helix region were defective for transformation of both fibroblasts and macrophages. Eight variants were cloned into the replication-competent avian expression vector RCAS. Analysis of cells infected with transformation-defective, replication-competent viruses confirmed the expression of functionally defective Myc proteins. Further, expression of the transformation defective variant dl91-137 in chicken fibroblasts inhibited subsequent transformation by wild-type MC29. The results reported herein support the hypothesis that Myc proteins function as regulators of transcription in a variety of cell types and clearly point out the necessity of putative regulatory domains within the amino-terminal half of the Myc protein.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-165881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-1840505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-1987636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-199997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2006410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-215208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2180585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2189105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2201910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2204813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2251503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2269425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-227607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2493990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2536982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2645525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2667136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-2668849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3018283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3028245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3041020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3054508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3289117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3295297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3299053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3438084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3467348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-366607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-3887132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-4290185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-4353520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6205983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6253823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6258948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6277501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6278322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6284967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6284968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6288259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6296857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6299767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6300896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6302688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6304354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6313208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6316149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6327075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6328273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6463648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6513926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313895-6656884
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2698-708
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1313895-Animals,
pubmed-meshheading:1313895-Mutation,
pubmed-meshheading:1313895-Chick Embryo,
pubmed-meshheading:1313895-Genetic Variation,
pubmed-meshheading:1313895-Fibroblasts,
pubmed-meshheading:1313895-Macrophages,
pubmed-meshheading:1313895-Cells, Cultured,
pubmed-meshheading:1313895-Avian leukosis virus,
pubmed-meshheading:1313895-Phenotype,
pubmed-meshheading:1313895-Cell Transformation, Viral,
pubmed-meshheading:1313895-Transcription, Genetic,
pubmed-meshheading:1313895-Cloning, Molecular,
pubmed-meshheading:1313895-Transfection,
pubmed-meshheading:1313895-Genetic Vectors,
pubmed-meshheading:1313895-Oncogene Protein p55(v-myc)
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