Linked Life Data

1313575

Source:http://linkedlifedata.com/resource/pubmed/id/1313575

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1992-5-6
pubmed:abstractText
The interaction of the immunodominant pertussis toxin peptide containing residues 30-42 (p30-42) with soluble DR1 molecules and the T-cell receptor (TCR) of 12 DR1-restricted human T-cell clones has been analyzed. Peptide analogues of p30-42 containing single alanine substitutions were used in DR1-binding and T-cell proliferation assays to identify the major histocompatibility complex and TCR contact residues. Each T-cell clone was found to recognize p30-42 with a different fine specificity. However, a common core comprising amino acids 33-39 was found to be important for stimulation of all T-cell clones. Within this core two residues, Leu33 and Leu36, interact with the DR1 molecule, whereas Asp34, His35, Thr37, and Arg39 are important for TCR recognition in most of the clones. Computer modeling of the structure of p30-42 showed that an alpha-helical conformation is compatible with the experimental data. The analysis of TCR rearrangement revealed that the peptide was recognized by T-cell clones expressing different variable region alpha (V alpha) and variable region beta (V beta) chains, although a preferential use of V alpha 8-V beta 13 and V alpha 11-V beta 18 combinations was found in clones from the same donor. Understanding the details of the interaction of antigenic peptides with the major histocompatibility complex and TCR molecules should provide the theoretical basis to design T-cell epitopes and obtain more immunogenic vaccines.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1690768, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1691261, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1693015, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1694219, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1695714, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1922337, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-1971424, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2141038, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2167910, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2189723, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2196996, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2199065, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2201750, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2295089, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2435001, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2435793, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2439915, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2452085, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2454990, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2455603, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2456857, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2460578, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2469443, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2469760, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2478291, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2478292, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2479705, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-2553848, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-3264322, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-3497349, http://linkedlifedata.com/resource/pubmed/commentcorrection/1313575-3876513
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2990-4
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed-meshheading:1313575-Amino Acid Sequence, pubmed-meshheading:1313575-Base Sequence, pubmed-meshheading:1313575-Binding Sites, pubmed-meshheading:1313575-Clone Cells, pubmed-meshheading:1313575-Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, pubmed-meshheading:1313575-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, pubmed-meshheading:1313575-HLA-DR1 Antigen, pubmed-meshheading:1313575-Humans, pubmed-meshheading:1313575-Models, Molecular, pubmed-meshheading:1313575-Molecular Sequence Data, pubmed-meshheading:1313575-Oligodeoxyribonucleotides, pubmed-meshheading:1313575-Peptides, pubmed-meshheading:1313575-Pertussis Toxin, pubmed-meshheading:1313575-Protein Binding, pubmed-meshheading:1313575-Receptors, Antigen, T-Cell, pubmed-meshheading:1313575-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:1313575-Virulence Factors, Bordetella
pubmed:year
1992
pubmed:articleTitle
Interaction of the pertussis toxin peptide containing residues 30-42 with DR1 and the T-cell receptors of 12 human T-cell clones.
pubmed:affiliation
Sclavo Research Center, Siena, Italy.
pubmed:publicationType
Journal Article, In Vitro